Intestinal Paneth cell differentiation relies on asymmetric
regulation of Wnt signaling by Daam1/2

Přehled o publikaci

J 2023

Intestinal Paneth cell differentiation relies on asymmetric regulation of Wnt signaling by Daam1/2

COLOZZA, Gabriele; Heetak LEE; Alessandra MERENDA; Sam Wu SZU-HSIEN; Andrea CATALÀ-BORDES et al.

Basic information

Original name

Intestinal Paneth cell differentiation relies on asymmetric regulation of Wnt signaling by Daam1/2

Authors

Edition

Science Advances, Washington, DC, American association for the advancement of science, 2023, 2375-2548

Other information

Language

English

Type of outcome

Article in a journal

Country of publisher

United States of America

Confidentiality degree

is not subject to a state or trade secret

References:

URL

Marked to be transferred to RIV

Yes

RIV identification code

RIV/00216224:14310/23:00132483

Organization

Přírodovědecká fakulta – Repository – Repository

Keywords in English

CONVERGENT EXTENSION; WNT/BETA-CATENIN; ENDOCYTOSIS; RECEPTORS; PROTEINS; ACTIVATION; GROWTH; LGR5; RHO

Links

GX19-28347X, research and development project. LM2023050, research and development project. LX22NPO5102, research and development project.

Changed: 26/4/2024 04:07, RNDr. Daniel Jakubík

Abstract

In the original language

The mammalian intestine is one of the most rapidly self-renewing tissues, driven by stem cells residing at the crypt bottom. Paneth cells form a major element of the niche microenvironment providing various growth factors to orchestrate intestinal stem cell homeostasis, such as Wnt3. Different Wnt ligands can selectively activate β-catenin–dependent (canonical) or –independent (noncanonical) signaling. Here, we report that the Dishevelled-associated activator of morphogenesis 1 (Daam1) and its paralogue Daam2 asymmetrically regulate canonical and noncanonical Wnt (Wnt/PCP) signaling. Daam1/2 interacts with the Wnt inhibitor RNF43, and Daam1/2 double knockout stimulates canonical Wnt signaling by preventing RNF43-dependent degradation of the Wnt receptor, Frizzled (Fzd). Single-cell RNA sequencing analysis revealed that Paneth cell differentiation is impaired by Daam1/2 depletion because of defective Wnt/PCP signaling. Together, we identified Daam1/2 as an unexpected hub molecule coordinating both canonical and noncanonical Wnt, which is fundamental for specifying an adequate number of Paneth cells.

Files attached

https://is.muni.cz/publication/2348565/Intestinal_Paneth_cell_differentiation_relies_on_asymmetric_regulation_of_Wnt_signaling_by_Daam1_2.pdf

Cite

COLOZZA, Gabriele; Heetak LEE; Alessandra MERENDA; Sam Wu SZU-HSIEN; Andrea CATALÀ-BORDES; Tomasz Witold RADASZKIEWICZ; Ingrid JORDENS; Lee JI-HYUN; Bamford AILEEN-DIANE; Fiona FARNHAMMER; Teck Yew LOW; Madelon M MAURICE; Vítězslav BRYJA; Jihoon KIM and Koo BON-KYOUNG. Intestinal Paneth cell differentiation relies on asymmetric regulation of Wnt signaling by Daam1/2. Science Advances. Washington, DC: American association for the advancement of science, 2023, vol. 9, No 47, p. 1-18. ISSN 2375-2548. Available from: https://doi.org/10.1126/sciadv.adh9673.

@article{58695,
   author = {Colozza, Gabriele and Lee, Heetak and Merenda, Alessandra and SzuandHsien, Sam Wu and CatalàandBordes, Andrea and Radaszkiewicz, Tomasz Witold and Jordens, Ingrid and JiandHyun, Lee and AileenandDiane, Bamford and Farnhammer, Fiona and Low, Teck Yew and Maurice, Madelon M and Bryja, Vítězslav and Kim, Jihoon and BonandKyoung, Koo},
   article_location = {Washington, DC},
   article_number = {47},
   doi = {https://doi.org/10.1126/sciadv.adh9673},
   keywords = {CONVERGENT EXTENSION; WNT/BETA-CATENIN; ENDOCYTOSIS; RECEPTORS; PROTEINS; ACTIVATION; GROWTH; LGR5; RHO},
   language = {eng},
   issn = {2375-2548},
   journal = {Science Advances},
   title = {Intestinal Paneth cell differentiation relies on asymmetric regulation of Wnt signaling by Daam1/2},
   url = {https://www.science.org/doi/10.1126/sciadv.adh9673},
   volume = {9},
   year = {2023}
}

TY  - JOUR
ID  - 58695
AU  - Colozza, Gabriele - Lee, Heetak - Merenda, Alessandra - Szu-Hsien, Sam Wu - Català-Bordes, Andrea - Radaszkiewicz, Tomasz Witold - Jordens, Ingrid - Ji-Hyun, Lee - Aileen-Diane, Bamford - Farnhammer, Fiona - Low, Teck Yew - Maurice, Madelon M - Bryja, Vítězslav - Kim, Jihoon - Bon-Kyoung, Koo
PY  - 2023
TI  - Intestinal Paneth cell differentiation relies on asymmetric regulation of Wnt signaling by Daam1/2
JF  - Science Advances
VL  - 9
IS  - 47
SP  - 1-18
EP  - 1-18
PB  - American association for the advancement of science
SN  - 2375-2548
KW  - CONVERGENT EXTENSION
KW  - WNT/BETA-CATENIN
KW  - ENDOCYTOSIS
KW  - RECEPTORS
KW  - PROTEINS
KW  - ACTIVATION
KW  - GROWTH
KW  - LGR5
KW  - RHO
UR  - https://www.science.org/doi/10.1126/sciadv.adh9673
N2  - The mammalian intestine is one of the most rapidly self-renewing tissues, driven by stem cells residing at the crypt bottom. Paneth cells form a major element of the niche microenvironment providing various growth factors to orchestrate intestinal stem cell homeostasis, such as Wnt3. Different Wnt ligands can selectively activate β-catenin–dependent (canonical) or –independent (noncanonical) signaling. Here, we report that the Dishevelled-associated activator of morphogenesis 1 (Daam1) and its paralogue Daam2 asymmetrically regulate canonical and noncanonical Wnt (Wnt/PCP) signaling. Daam1/2 interacts with the Wnt inhibitor RNF43, and Daam1/2 double knockout stimulates canonical Wnt signaling by preventing RNF43-dependent degradation of the Wnt receptor, Frizzled (Fzd). Single-cell RNA sequencing analysis revealed that Paneth cell differentiation is impaired by Daam1/2 depletion because of defective Wnt/PCP signaling. Together, we identified Daam1/2 as an unexpected hub molecule coordinating both canonical and noncanonical Wnt, which is fundamental for specifying an adequate number of Paneth cells.
ER  -

COLOZZA, Gabriele; Heetak LEE; Alessandra MERENDA; Sam Wu SZU-HSIEN; Andrea CATALÀ-BORDES; Tomasz Witold RADASZKIEWICZ; Ingrid JORDENS; Lee JI-HYUN; Bamford AILEEN-DIANE; Fiona FARNHAMMER; Teck Yew LOW; Madelon M MAURICE; Vítězslav BRYJA; Jihoon KIM and Koo BON-KYOUNG. Intestinal Paneth cell differentiation relies on asymmetric regulation of Wnt signaling by Daam1/2. \textit{Science Advances}. Washington, DC: American association for the advancement of science, 2023, vol.~9, No~47, p.~1-18. ISSN~2375-2548. Available from: https://doi.org/10.1126/sciadv.adh9673.

Přehled o publikaci