Cerebral organoids derived from patients with Alzheimer´s
disease with PSEN1/2 mutations have defective tissue patterning
and altered development

Přehled o publikaci

J 2023

Cerebral organoids derived from patients with Alzheimer´s disease with PSEN1/2 mutations have defective tissue patterning and altered development

VÁŇOVÁ, Tereza; Jiří SEDMÍK; Jan RAŠKA; Kateřina AMRUZ ČERNÁ; Petr TAUŠ et al.

Základní údaje

Originální název

Cerebral organoids derived from patients with Alzheimer´s disease with PSEN1/2 mutations have defective tissue patterning and altered development

Autoři

Vydání

CELL REPORTS, UNITED STATES, CELL PRESS, 2023, 2211-1247

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/23:00132029

Organizace

Lékařská fakulta – Masarykova univerzita – Repozitář

Klíčová slova anglicky

Cerebral organoids; Alzheimer's disease; PSEN1/2 mutations

Návaznosti

CZ.02.1.01/0.0/0.0/16_013/0001761, interní kód Repo. EF15_003/0000469, projekt VaV. EF16_013/0001761, projekt VaV. EF17_043/0009632, projekt VaV. EF19_073/0016943, projekt VaV. GA20-15728S, projekt VaV. GA21-21510S, projekt VaV. LX22NPO5107, projekt VaV. MUNI/A/1301/2022, interní kód Repo. MUNI/G/1131/2017, interní kód Repo. MUNI/IGA/1273/2021, interní kód Repo. MUNI/R/1321/2021, interní kód Repo. MUNI/R/1697/2020, interní kód Repo. NU20-08-00314, projekt VaV. NU21-08-00373, projekt VaV. NU22J-08-00075, projekt VaV. NU22-04-00366, projekt VaV. NV19-08-00472, projekt VaV. 101087124, interní kód Repo. 8F20009, projekt VaV. 857560, interní kód Repo. RECETOX RI, velká výzkumná infrastruktura. Czech-BioImaging II, velká výzkumná infrastruktura. NCMG II, velká výzkumná infrastruktura.

Změněno: 17. 1. 2025 00:50, RNDr. Daniel Jakubík

Anotace

V originále

During the past two decades, induced pluripotent stem cells (iPSCs) have been widely used to study human neural development and disease. Especially in the field of Alzheimer’s disease (AD), remarkable effort has been put into investigating molecular mechanisms behind this disease. Then, with the advent of 3D neuronal cultures and cerebral organoids (COs), several studies have demonstrated that this model can adequately mimic familial and sporadic AD. Therefore, we created an AD-CO model using iPSCs derived from patients with familial AD forms and explored early events and the progression of AD pathogenesis. Our study demonstrated that COs derived from three AD-iPSC lines with PSEN1(A246E) or PSEN2(N141I) mutations developed the AD-specific markers in vitro, yet they also uncover tissue patterning defects and altered development. These findings are complemented by single-cell sequencing data confirming this observation and uncovering that neurons in AD-COs likely differentiate prematurely.

Přiložené soubory

https://is.muni.cz/publication/2329980/2329980.pdf

Citovat

VÁŇOVÁ, Tereza; Jiří SEDMÍK; Jan RAŠKA; Kateřina AMRUZ ČERNÁ; Petr TAUŠ; Veronika POSPÍŠILOVÁ; Markéta NEZVEDOVÁ; Veronika FEDOROVÁ; Soňa KADÁKOVÁ; Hana KLÍMOVÁ; Michaela CAPANDOVÁ; Petra ORVISKÁ; Petr FOJTÍK; Simona BÁRTOVÁ; Karla PLEVOVÁ; Zdeněk SPÁČIL; Hana HŘÍBKOVÁ a Dáša BOHAČIAKOVÁ. Cerebral organoids derived from patients with Alzheimer´s disease with PSEN1/2 mutations have defective tissue patterning and altered development. CELL REPORTS. UNITED STATES: CELL PRESS, 2023, roč. 42, č. 11, s. 1-27. ISSN 2211-1247. Dostupné z: https://doi.org/10.1016/j.celrep.2023.113310.

@article{58073,
   author = {Váňová, Tereza and Sedmík, Jiří and Raška, Jan and Amruz Černá, Kateřina and Tauš, Petr and Pospíšilová, Veronika and Nezvedová, Markéta and Fedorová, Veronika and Kadáková, Soňa and Klímová, Hana and Capandová, Michaela and Orviská, Petra and Fojtík, Petr and Bártová, Simona and Plevová, Karla and Spáčil, Zdeněk and Hříbková, Hana and Bohačiaková, Dáša},
   article_location = {UNITED STATES},
   article_number = {11},
   doi = {https://doi.org/10.1016/j.celrep.2023.113310},
   keywords = {Cerebral organoids; Alzheimer's disease; PSEN1/2 mutations},
   language = {eng},
   issn = {2211-1247},
   journal = {CELL REPORTS},
   title = {Cerebral organoids derived from patients with Alzheimer´s disease with PSEN1/2 mutations have defective tissue patterning and altered development},
   url = {https://www.sciencedirect.com/science/article/pii/S2211124723013220?via%3Dihub},
   volume = {42},
   year = {2023}
}

TY  - JOUR
ID  - 58073
AU  - Váňová, Tereza - Sedmík, Jiří - Raška, Jan - Amruz Černá, Kateřina - Tauš, Petr - Pospíšilová, Veronika - Nezvedová, Markéta - Fedorová, Veronika - Kadáková, Soňa - Klímová, Hana - Capandová, Michaela - Orviská, Petra - Fojtík, Petr - Bártová, Simona - Plevová, Karla - Spáčil, Zdeněk - Hříbková, Hana - Bohačiaková, Dáša
PY  - 2023
TI  - Cerebral organoids derived from patients with Alzheimer´s disease with PSEN1/2 mutations have defective tissue patterning and altered development
JF  - CELL REPORTS
VL  - 42
IS  - 11
SP  - 1-27
EP  - 1-27
PB  - CELL PRESS
SN  - 2211-1247
KW  - Cerebral organoids
KW  - Alzheimer's disease
KW  - PSEN1/2 mutations
UR  - https://www.sciencedirect.com/science/article/pii/S2211124723013220?via%3Dihub
N2  - During the past two decades, induced pluripotent stem cells (iPSCs) have been widely used to study human neural development and disease. Especially in the field of Alzheimer’s disease (AD), remarkable effort has been put into investigating molecular mechanisms behind this disease. Then, with the advent of 3D neuronal cultures and cerebral organoids (COs), several studies have demonstrated that this model can adequately mimic familial and sporadic AD. Therefore, we created an AD-CO model using iPSCs derived from patients with familial AD forms and explored early events and the progression of AD pathogenesis. Our study demonstrated that COs derived from three AD-iPSC lines with PSEN1(A246E) or PSEN2(N141I) mutations developed the AD-specific markers in vitro, yet they also uncover tissue patterning defects and altered development. These findings are complemented by single-cell sequencing data confirming this observation and uncovering that neurons in AD-COs likely differentiate prematurely.
ER  -

VÁŇOVÁ, Tereza; Jiří SEDMÍK; Jan RAŠKA; Kateřina AMRUZ ČERNÁ; Petr TAUŠ; Veronika POSPÍŠILOVÁ; Markéta NEZVEDOVÁ; Veronika FEDOROVÁ; Soňa KADÁKOVÁ; Hana KLÍMOVÁ; Michaela CAPANDOVÁ; Petra ORVISKÁ; Petr FOJTÍK; Simona BÁRTOVÁ; Karla PLEVOVÁ; Zdeněk SPÁČIL; Hana HŘÍBKOVÁ a Dáša BOHAČIAKOVÁ. Cerebral organoids derived from patients with Alzheimer´s disease with PSEN1/2 mutations have defective tissue patterning and altered development. \textit{CELL REPORTS}. UNITED STATES: CELL PRESS, 2023, roč.~42, č.~11, s.~1-27. ISSN~2211-1247. Dostupné z: https://doi.org/10.1016/j.celrep.2023.113310.

Přehled o publikaci