Přehled o publikaci
2023
Cerebral organoids derived from patients with Alzheimer´s disease with PSEN1/2 mutations have defective tissue patterning and altered development
VÁŇOVÁ, Tereza; Jiří SEDMÍK; Jan RAŠKA; Kateřina AMRUZ ČERNÁ; Petr TAUŠ et. al.Basic information
Original name
Cerebral organoids derived from patients with Alzheimer´s disease with PSEN1/2 mutations have defective tissue patterning and altered development
Authors
VÁŇOVÁ, Tereza (203 Czech Republic, belonging to the institution); Jiří SEDMÍK (203 Czech Republic, belonging to the institution); Jan RAŠKA (203 Czech Republic, belonging to the institution); Kateřina AMRUZ ČERNÁ (203 Czech Republic, belonging to the institution); Petr TAUŠ (203 Czech Republic, belonging to the institution); Veronika POSPÍŠILOVÁ (203 Czech Republic, belonging to the institution); Markéta NEZVEDOVÁ (203 Czech Republic, belonging to the institution); Veronika FEDOROVÁ (203 Czech Republic, belonging to the institution); Soňa KADÁKOVÁ (703 Slovakia, belonging to the institution); Hana KLÍMOVÁ (203 Czech Republic, belonging to the institution); Michaela CAPANDOVÁ (203 Czech Republic, belonging to the institution); Petra ORVISKÁ (703 Slovakia, belonging to the institution); Petr FOJTÍK (203 Czech Republic, belonging to the institution); Simona BÁRTOVÁ (203 Czech Republic, belonging to the institution); Karla PLEVOVÁ (203 Czech Republic, belonging to the institution); Zdeněk SPÁČIL (203 Czech Republic, belonging to the institution); Hana HŘÍBKOVÁ (203 Czech Republic, belonging to the institution) and Dáša BOHAČIAKOVÁ (703 Slovakia, guarantor, belonging to the institution)
Edition
CELL REPORTS, UNITED STATES, CELL PRESS, 2023, 2211-1247
Other information
Language
English
Type of outcome
Article in a journal
Country of publisher
United States of America
Confidentiality degree
is not subject to a state or trade secret
References:
RIV identification code
RIV/00216224:14110/23:00132029
Organization
Lékařská fakulta – Repository – Repository
UT WoS
001096821600001
EID Scopus
2-s2.0-85174468116
Keywords in English
Cerebral organoids; Alzheimer's disease; PSEN1/2 mutations
Links
CZ.02.1.01/0.0/0.0/16_013/0001761, interní kód Repo. EF15_003/0000469, research and development project. EF16_013/0001761, research and development project. EF17_043/0009632, research and development project. EF19_073/0016943, research and development project. GA20-15728S, research and development project. GA21-21510S, research and development project. LX22NPO5107, research and development project. MUNI/A/1301/2022, interní kód Repo. MUNI/G/1131/2017, interní kód Repo. MUNI/IGA/1273/2021, interní kód Repo. MUNI/R/1321/2021, interní kód Repo. MUNI/R/1697/2020, interní kód Repo. NU20-08-00314, research and development project. NU21-08-00373, research and development project. NU22J-08-00075, research and development project. NU22-04-00366, research and development project. NV19-08-00472, research and development project. 101087124, interní kód Repo. 8F20009, research and development project. 857560, interní kód Repo. RECETOX RI, large research infrastructures. Czech-BioImaging II, large research infrastructures. NCMG II, large research infrastructures.
Changed: 17/1/2025 00:50, RNDr. Daniel Jakubík
Abstract
V originále
During the past two decades, induced pluripotent stem cells (iPSCs) have been widely used to study human neural development and disease. Especially in the field of Alzheimer’s disease (AD), remarkable effort has been put into investigating molecular mechanisms behind this disease. Then, with the advent of 3D neuronal cultures and cerebral organoids (COs), several studies have demonstrated that this model can adequately mimic familial and sporadic AD. Therefore, we created an AD-CO model using iPSCs derived from patients with familial AD forms and explored early events and the progression of AD pathogenesis. Our study demonstrated that COs derived from three AD-iPSC lines with PSEN1(A246E) or PSEN2(N141I) mutations developed the AD-specific markers in vitro, yet they also uncover tissue patterning defects and altered development. These findings are complemented by single-cell sequencing data confirming this observation and uncovering that neurons in AD-COs likely differentiate prematurely.
