Detailed Information on Publication Record
2019
Label-free Voltammetric Detection of Products of Terminal Deoxynucleotidyl Transferase Tailing Reaction
HERMANOVA, M., P. HAVRANOVA-VIDLAKOVA, A. ONDRACKOVA, S.S. KUMAR, R. BOWATER et. al.Basic information
Original name
Label-free Voltammetric Detection of Products of Terminal Deoxynucleotidyl Transferase Tailing Reaction
Authors
HERMANOVA, M. (203 Czech Republic), P. HAVRANOVA-VIDLAKOVA (203 Czech Republic), A. ONDRACKOVA (203 Czech Republic), S.S. KUMAR (826 United Kingdom of Great Britain and Northern Ireland), R. BOWATER (826 United Kingdom of Great Britain and Northern Ireland) and Miroslav FOJTA (203 Czech Republic, guarantor, belonging to the institution)
Edition
Electroanalysis, WEINHEIM, WILEY-VCH Verlag GmbH, 2019, 1040-0397
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Country of publisher
Germany
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
RIV identification code
RIV/00216224:14740/19:00107289
Organization
Středoevropský technologický institut – Repository – Repository
UT WoS
000459630500012
Keywords in English
terminal deoxynucleotidyl transferase; oligonucleotide tailing; DNA electrochemistry; label free; nucleobase; reduction; oxidation
Links
GBP206/12/G151, research and development project. LQ1601, research and development project. 692068, interní kód Repo.
Změněno: 7/9/2020 18:11, RNDr. Daniel Jakubík
Abstract
V originále
A label-free approach that takes advantage of intrinsic electrochemical activity of nucleobases has been applied to study the products of terminal deoxynucleotidyl transferase (TdT) tailing reaction. DNA homooligonucleotides A(30), C-30 and T-30 were used as primers for the tailing reaction to which a dNTP - or a mixture of dNTPs - and TdT were added to form the tails. Electrochemical detection enabled study of the tailing reaction products created by various combinations of primers and dNTPs, with pyrolytic graphite electrode (PGE) being suitable for remarkably precise analysis of the length of tailing reaction products. Furthermore, the hanging mercury drop electrode (HMDE) was able to reveal formation of various DNA structures, such as DNA hairpins and G-quadruplexes, which influence the behavior of DNA molecules at the negatively charged surface of HMDE. Thus, the described approach proves to be an excellent tool for studying the TdT tailing reactions and for exploring how various DNA structures affect both the tailing reactions and electrochemical behavior of DNA oligonucleotides at electrode surfaces.