Reliable detection of CNS lymphoma-derived circulating tumor
DNA in cerebrospinal fluid using multi-biomarker NGS profiling:
insights from a real-world study

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Reliable detection of CNS lymphoma-derived circulating tumor DNA in cerebrospinal fluid using ...

Přehled o publikaci

J 2025

Reliable detection of CNS lymphoma-derived circulating tumor DNA in cerebrospinal fluid using multi-biomarker NGS profiling: insights from a real-world study

NAVRKALOVÁ, Veronika, Andrea MAREČKOVÁ, Samuel HRICKO, Viera HRABČÁKOVÁ, Lenka RADOVÁ et. al.

Základní údaje

Originální název

Reliable detection of CNS lymphoma-derived circulating tumor DNA in cerebrospinal fluid using multi-biomarker NGS profiling: insights from a real-world study

Autoři

Vydání

BIOMARKER RESEARCH, LONDON, BMC, 2025, 2050-7771

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Organizace

Lékařská fakulta – Masarykova univerzita – Repozitář

DOI

http://dx.doi.org/10.1186/s40364-025-00777-z

UT WoS

001485606600001

EID Scopus

2-s2.0-105004890394

Klíčová slova anglicky

ctDNA detection; Liquid biopsy; Multi-target NGS; Clinical practice; CNS lymphomas

Návaznosti

LM2018132, projekt VaV. LM2023067, projekt VaV. LX22NPO5102, projekt VaV. LX22NPO5107, projekt VaV. MUNI/A/1685/2024, interní kód Repo. NU22-08-00227, projekt VaV. e-INFRA CZ II, velká výzkumná infrastruktura.

Změněno: 30. 5. 2025 00:50, RNDr. Daniel Jakubík

Anotace

V originále

lt; 0.001). Parallel analysis of tumor DNA in both cell-free and cellular DNA from CSF makes the probability of primary or secondary CNS malignancy detection even higher. Conclusions Our prospective, tissue-agnostic approach highlights the feasibility of ctDNA sequencing by a commonplace and affordable method, offering higher sensitivity to detect CNS infiltration with lymphoma than standard cell-analyzing techniques. We accentuate the benefit of a multi-target NGS approach and adequate CSF sampling to obtain satisfactory diagnostic yield. Less invasive liquid biopsy testing by comprehensive NGS complements standard procedures in the diagnostics and management of CNSL patients, especially when encountering limitations.

Přiložené soubory

https://is.muni.cz/publication/2499805/Reliable_detection_of_CNS_lymphoma-derived_circulating_tumor_DNA_in_cerebrospinal_fluid_using_multi-biomarker_NGS_profiling_insights_from_a_real-world_study.pdf

Citovat

NAVRKALOVÁ, Veronika, Andrea MAREČKOVÁ, Samuel HRICKO, Viera HRABČÁKOVÁ, Lenka RADOVÁ, Václav KUBEŠ, Jakub Paweł PORC, Tomáš REIGL, Šárka POSPÍŠILOVÁ, Jana KOTAŠKOVÁ a Andrea JANÍKOVÁ. Reliable detection of CNS lymphoma-derived circulating tumor DNA in cerebrospinal fluid using multi-biomarker NGS profiling: insights from a real-world study. BIOMARKER RESEARCH. LONDON: BMC, 2025, roč. 13, č. 1, s. 1-12. ISSN 2050-7771. Dostupné z: https://dx.doi.org/10.1186/s40364-025-00777-z.

@article{74378,
   author = {Navrkalová, Veronika and Marečková, Andrea and Hricko, Samuel and Hrabčáková, Viera and Radová, Lenka and Kubeš, Václav and Porc, Jakub Paweł and Reigl, Tomáš and Pospíšilová, Šárka and Kotašková, Jana and Janíková, Andrea},
   article_location = {LONDON},
   article_number = {1},
   doi = {http://dx.doi.org/10.1186/s40364-025-00777-z},
   keywords = {ctDNA detection; Liquid biopsy; Multi-target NGS; Clinical practice; CNS lymphomas},
   language = {eng},
   issn = {2050-7771},
   journal = {BIOMARKER RESEARCH},
   title = {Reliable detection of CNS lymphoma-derived circulating tumor DNA in cerebrospinal fluid using multi-biomarker NGS profiling: insights from a real-world study},
   url = {https://biomarkerres.biomedcentral.com/articles/10.1186/s40364-025-00777-z},
   volume = {13},
   year = {2025}
}

TY  - JOUR
ID  - 74378
AU  - Navrkalová, Veronika - Marečková, Andrea - Hricko, Samuel - Hrabčáková, Viera - Radová, Lenka - Kubeš, Václav - Porc, Jakub Paweł - Reigl, Tomáš - Pospíšilová, Šárka - Kotašková, Jana - Janíková, Andrea
PY  - 2025
TI  - Reliable detection of CNS lymphoma-derived circulating tumor DNA in cerebrospinal fluid using multi-biomarker NGS profiling: insights from a real-world study
JF  - BIOMARKER RESEARCH
VL  - 13
IS  - 1
SP  - 1-12
EP  - 1-12
PB  - BMC
SN  - 2050-7771
KW  - ctDNA detection
KW  - Liquid biopsy
KW  - Multi-target NGS
KW  - Clinical practice
KW  - CNS lymphomas
UR  - https://biomarkerres.biomedcentral.com/articles/10.1186/s40364-025-00777-z
N2  - lt; 0.001). Parallel analysis of tumor DNA in both cell-free and cellular DNA from CSF makes the probability of primary or secondary CNS malignancy detection even higher. Conclusions Our prospective, tissue-agnostic approach highlights the feasibility of ctDNA sequencing by a commonplace and affordable method, offering higher sensitivity to detect CNS infiltration with lymphoma than standard cell-analyzing techniques. We accentuate the benefit of a multi-target NGS approach and adequate CSF sampling to obtain satisfactory diagnostic yield. Less invasive liquid biopsy testing by comprehensive NGS complements standard procedures in the diagnostics and management of CNSL patients, especially when encountering limitations.
ER  -

NAVRKALOVÁ, Veronika, Andrea MAREČKOVÁ, Samuel HRICKO, Viera HRABČÁKOVÁ, Lenka RADOVÁ, Václav KUBEŠ, Jakub Paweł PORC, Tomáš REIGL, Šárka POSPÍŠILOVÁ, Jana KOTAŠKOVÁ a Andrea JANÍKOVÁ. Reliable detection of CNS lymphoma-derived circulating tumor DNA in cerebrospinal fluid using multi-biomarker NGS profiling: insights from a real-world study. \textit{BIOMARKER RESEARCH}. LONDON: BMC, 2025, roč.~13, č.~1, s.~1-12. ISSN~2050-7771. Dostupné z: https://dx.doi.org/10.1186/s40364-025-00777-z.