a 2024

Seed and embryo imaging: what works and what doesn't

ROBERT BOISIVON, Helene

Basic information

Original name

Seed and embryo imaging: what works and what doesn't

Authors

ROBERT BOISIVON, Helene

Edition

RECROP, workshop Portici, 2024: “Molecular Insights into Stress-Resilient Crop Reproduction and Yield” 2024

Other information

Language

English

Type of outcome

Konferenční abstrakta

Country of publisher

Italy

Confidentiality degree

is not subject to a state or trade secret

References:

Marked to be transferred to RIV

Yes

RIV identification code

RIV/00216224:14740/24:00138956

Organization

Středoevropský technologický institut – Repository – Repository

Keywords in English

climate changes; seed production; Arabidopsis thaliana; Brassica napus; tomato; seed imaging methods

Links

EH22_008/0004581, research and development project.
Changed: 26/4/2025 00:50, RNDr. Daniel Jakubík

Abstract

In the original language

Flowering plants produce seeds, one of the bases of our food. However, instances of climate change with increased temperatures and the occurrence of heat waves deeply affect seed production. In this lecture, I will present different imaging technologies to assess resilience to stress during seed development. I will catalogue the different imaging methodologies used in our laboratory to assess seed and embryo development in Arabidopsis thaliana, Brassica napus and tomato. Seeds are imaged for morphological assessment or assessment of biosensors. It requires specific equipment such as light, fluorescence, confocal microscopes, or X-ray computer tomography. Samples may have to be prepared (fixed and sectioned, cleared). Examples of what works and what does not work in our hands will be provided.

Files attached