Přehled o publikaci
2025
Automated Sequential Derivatization for Gas Chromatography-[Orbitrap] Mass Spectrometry-based Metabolite Profiling of Human Blood-based Samples
JBEBLI, Akrem, Kateřina COUFALÍKOVÁ, Moira ZANABONI, Manuela BERGNA, Renzo PICENONI et. al.Basic information
Original name
Automated Sequential Derivatization for Gas Chromatography-[Orbitrap] Mass Spectrometry-based Metabolite Profiling of Human Blood-based Samples
Authors
JBEBLI, Akrem, Kateřina COUFALÍKOVÁ, Moira ZANABONI, Manuela BERGNA, Renzo PICENONI, Jana KLÁNOVÁ and Elliott James PRICE
Edition
Bio-protocol, SUNNYVALE, BIO-PROTOCOL, 2025, 2331-8325
Other information
Language
English
Type of outcome
Article in a journal
Country of publisher
United States of America
Confidentiality degree
is not subject to a state or trade secret
Organization
Přírodovědecká fakulta – Repository – Repository
UT WoS
001441429500002
EID Scopus
2-s2.0-86000350294
Keywords in English
Automation; Derivatization; Thermo ScientificTM TriPlusTM RSH; Metabolite profiling; Gas chromatography-mass spectrometry (GC-MS)
Links
EH22_008/0004644, research and development project. LM2023069, research and development project. 857560, interní kód Repo.
Changed: 28/5/2025 00:50, RNDr. Daniel Jakubík
Abstract
V originále
Many small molecules require derivatization to increase their volatility and to be amenable to gas chromatographic (GC) separation. Derivatization is usually time-consuming, and typical batch-wise procedures increase sample variability. Sequential automation of derivatization via robotic liquid handling enables the overlapping of sample preparation and analysis, maximizing time efficiency and minimizing variability. Herein, a protocol for the fully automated, two-stage derivatization of human blood-based samples in line with GC- [Orbitrap] mass spectrometry (MS)-based metabolomics is described. The protocol delivers a sample-to-sample runtime of 31 min, being suitable for better throughput routine metabolomic analysis.
