HORKÁ, Marie, Filip RŮŽIČKA, Alena SIVÁKOVÁ, Pavel KARÁSEK, Jiří ŠALPLACHTA, Roman PANTŮČEK and Michal ROTH. Capillary electrophoretic methods for classification of methicillin-resistant Staphylococcus aureus (MRSA) clones. Analytica Chimica Acta. Amsterdam: Elsevier Science publishers, 2022, vol. 1227, September, p. 1-7. ISSN 0003-2670. Available from: https://dx.doi.org/10.1016/j.aca.2022.340305.
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Basic information
Original name Capillary electrophoretic methods for classification of methicillin-resistant Staphylococcus aureus (MRSA) clones
Authors HORKÁ, Marie (203 Czech Republic), Filip RŮŽIČKA (203 Czech Republic, guarantor, belonging to the institution), Alena SIVÁKOVÁ (203 Czech Republic, belonging to the institution), Pavel KARÁSEK (203 Czech Republic), Jiří ŠALPLACHTA (203 Czech Republic), Roman PANTŮČEK (203 Czech Republic, belonging to the institution) and Michal ROTH (203 Czech Republic).
Edition Analytica Chimica Acta, Amsterdam, Elsevier Science publishers, 2022, 0003-2670.
Other information
Original language English
Type of outcome Article in a journal
Country of publisher Netherlands
Confidentiality degree is not subject to a state or trade secret
WWW URL
RIV identification code RIV/00216224:14310/22:00126934
Organization Přírodovědecká fakulta – Repository – Repository
Doi http://dx.doi.org/10.1016/j.aca.2022.340305
UT WoS 000862946300005
Keywords in English Capillary electrophoresis; Classification; Fused silica capillary; Separation; Staphylococcus aureus; Temperature; MRSA
Links LX22NPO5103, research and development project. NU22-05-00110, research and development project.
Changed by Changed by: RNDr. Daniel Jakubík, učo 139797. Changed: 6/3/2024 03:38.
Abstract
This study describes differentiation of methicillin-resistant Staphylococcus aureus (MRSA) isolates belonging to different genotype groups by the combination of electrophoretic techniques, transient isotachophoresis and micellar electrokinetic chromatography. MRSA isolates were separated in fused silica capillary with roughened inner surface prepared by etching with supercritical water. Separation temperature together with the rinsing procedure of the capillary turned out to be the key factors of successful analysis. The individual genotype groups were baseline-resolved in 40 min. Partial separation of the individual isolates within the groups was also observed. Relative standard deviations of the migration times of the isolate zones ranged from 0.32 to 0.79%. In addition, capability of the developed CE method to concentrate and separate MRSA isolates in clinical samples was proved by the analysis of blood sample.
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  • a concrete person Mgr. Michal Maňas, uco 2481
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