HORKÁ, Marie, Filip RŮŽIČKA, Alena SIVÁKOVÁ, Pavel KARÁSEK, Jiří ŠALPLACHTA, Roman PANTŮČEK and Michal ROTH. Capillary electrophoretic methods for classification of methicillin-resistant Staphylococcus aureus (MRSA) clones. Analytica Chimica Acta. Amsterdam: Elsevier Science publishers, 2022, vol. 1227, September, p. 1-7. ISSN 0003-2670. Available from: https://dx.doi.org/10.1016/j.aca.2022.340305. |
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@article{60006, author = {Horká, Marie and Růžička, Filip and Siváková, Alena and Karásek, Pavel and Šalplachta, Jiří and Pantůček, Roman and Roth, Michal}, article_location = {Amsterdam}, article_number = {September}, doi = {http://dx.doi.org/10.1016/j.aca.2022.340305}, keywords = {Capillary electrophoresis; Classification; Fused silica capillary; Separation; Staphylococcus aureus; Temperature; MRSA}, language = {eng}, issn = {0003-2670}, journal = {Analytica Chimica Acta}, title = {Capillary electrophoretic methods for classification of methicillin-resistant Staphylococcus aureus (MRSA) clones}, url = {https://doi.org/10.1016/j.aca.2022.340305}, volume = {1227}, year = {2022} }
TY - JOUR ID - 60006 AU - Horká, Marie - Růžička, Filip - Siváková, Alena - Karásek, Pavel - Šalplachta, Jiří - Pantůček, Roman - Roth, Michal PY - 2022 TI - Capillary electrophoretic methods for classification of methicillin-resistant Staphylococcus aureus (MRSA) clones JF - Analytica Chimica Acta VL - 1227 IS - September SP - 1-7 EP - 1-7 PB - Elsevier Science publishers SN - 0003-2670 KW - Capillary electrophoresis KW - Classification KW - Fused silica capillary KW - Separation KW - Staphylococcus aureus KW - Temperature KW - MRSA UR - https://doi.org/10.1016/j.aca.2022.340305 N2 - This study describes differentiation of methicillin-resistant Staphylococcus aureus (MRSA) isolates belonging to different genotype groups by the combination of electrophoretic techniques, transient isotachophoresis and micellar electrokinetic chromatography. MRSA isolates were separated in fused silica capillary with roughened inner surface prepared by etching with supercritical water. Separation temperature together with the rinsing procedure of the capillary turned out to be the key factors of successful analysis. The individual genotype groups were baseline-resolved in 40 min. Partial separation of the individual isolates within the groups was also observed. Relative standard deviations of the migration times of the isolate zones ranged from 0.32 to 0.79%. In addition, capability of the developed CE method to concentrate and separate MRSA isolates in clinical samples was proved by the analysis of blood sample. ER -
HORKÁ, Marie, Filip RŮŽIČKA, Alena SIVÁKOVÁ, Pavel KARÁSEK, Jiří ŠALPLACHTA, Roman PANTŮČEK and Michal ROTH. Capillary electrophoretic methods for classification of methicillin-resistant Staphylococcus aureus (MRSA) clones. \textit{Analytica Chimica Acta}. Amsterdam: Elsevier Science publishers, 2022, vol.~1227, September, p.~1-7. ISSN~0003-2670. Available from: https://dx.doi.org/10.1016/j.aca.2022.340305.
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