Přehled o publikaci
2022
Capillary electrophoretic methods for classification of methicillin-resistant Staphylococcus aureus (MRSA) clones
HORKÁ, Marie; Filip RŮŽIČKA; Alena SIVÁKOVÁ; Pavel KARÁSEK; Jiří ŠALPLACHTA et al.Basic information
Original name
Capillary electrophoretic methods for classification of methicillin-resistant Staphylococcus aureus (MRSA) clones
Authors
HORKÁ, Marie; Filip RŮŽIČKA; Alena SIVÁKOVÁ; Pavel KARÁSEK; Jiří ŠALPLACHTA; Roman PANTŮČEK and Michal ROTH
Edition
Analytica Chimica Acta, Amsterdam, Elsevier Science publishers, 2022, 0003-2670
Other information
Language
English
Type of outcome
Article in a journal
Country of publisher
Netherlands
Confidentiality degree
is not subject to a state or trade secret
References:
Marked to be transferred to RIV
Yes
RIV identification code
RIV/00216224:14310/22:00126934
Organization
Přírodovědecká fakulta – Repository – Repository
UT WoS
EID Scopus
Keywords in English
Capillary electrophoresis; Classification; Fused silica capillary; Separation; Staphylococcus aureus; Temperature; MRSA
Links
LX22NPO5103, research and development project. NU22-05-00110, research and development project.
Changed: 6/3/2024 03:38, RNDr. Daniel Jakubík
Abstract
In the original language
This study describes differentiation of methicillin-resistant Staphylococcus aureus (MRSA) isolates belonging to different genotype groups by the combination of electrophoretic techniques, transient isotachophoresis and micellar electrokinetic chromatography. MRSA isolates were separated in fused silica capillary with roughened inner surface prepared by etching with supercritical water. Separation temperature together with the rinsing procedure of the capillary turned out to be the key factors of successful analysis. The individual genotype groups were baseline-resolved in 40 min. Partial separation of the individual isolates within the groups was also observed. Relative standard deviations of the migration times of the isolate zones ranged from 0.32 to 0.79%. In addition, capability of the developed CE method to concentrate and separate MRSA isolates in clinical samples was proved by the analysis of blood sample.
