Generation of the Human iPSC Line from Spontaneous Late-Onset
Alzheimer's Disease Patient with ApoE3/3 Genotype and Sex-,
Age-, and ApoE-Matched Healthy Control

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Generation of the Human iPSC Line from Spontaneous Late-Onset Alzheimer's Disease Patient with ...

Přehled o publikaci

J 2024

Generation of the Human iPSC Line from Spontaneous Late-Onset Alzheimer's Disease Patient with ApoE3/3 Genotype and Sex-, Age-, and ApoE-Matched Healthy Control

KADÁKOVÁ, Soňa; Jan RAŠKA; Hana HŘÍBKOVÁ; Veronika FEDOROVÁ; Jiří SEDMÍK et. al.

Basic information

Original name

Generation of the Human iPSC Line from Spontaneous Late-Onset Alzheimer's Disease Patient with ApoE3/3 Genotype and Sex-, Age-, and ApoE-Matched Healthy Control

Authors

KADÁKOVÁ, Soňa; Jan RAŠKA; Hana HŘÍBKOVÁ; Veronika FEDOROVÁ; Jiří SEDMÍK; Klára PLEŠINGROVÁ; Kateřina SHEARDOVÁ and Dáša BOHAČIAKOVÁ

Edition

STEM CELL RESEARCH, AMSTERDAM, ELSEVIER, 2024, 1873-5061

Other information

Language

English

Type of outcome

Article in a journal

Country of publisher

Netherlands

Confidentiality degree

is not subject to a state or trade secret

References:

URL

Organization

Lékařská fakulta – Repository – Repository

DOI

http://dx.doi.org/10.1016/j.scr.2023.103273

UT WoS

001160676800001

EID Scopus

2-s2.0-85179661891

Keywords in English

Human iPSC Line; Alzheimer’s Disease; ApoE3/3 Genotype

Links

GA21-21510S, research and development project. LM2018129, research and development project. LX22NPO5107, research and development project. MUNI/A/1301/2022, interní kód Repo. NU21-08-00373, research and development project. 101087124, interní kód Repo.

Changed: 3/10/2024 00:50, RNDr. Daniel Jakubík

Abstract

V originále

Human induced pluripotent stem cell (iPSC) lines were generated from peripheral blood mononuclear cells (PBMCs) isolated from a patient diagnosed with spontaneous late -onset Alzheimer's disease (AD) carrying ApoE3/3 gene and one age-, sex-, and ApoE-matched healthy control. Reprogramming was done using a commercially available Epi5 Reprogramming Kit containing OCT4, SOX2, KLF4, LIN28, and L-MYC as reprogramming factors. The pluripotency of the iPSC lines was verified by the expression of pluripotency markers and by their capacity to differentiate into all three embryonic germ layers in vitro. These newly established iPSC lines offer a valuable platform for in vitro modeling of AD.

Files attached

https://is.muni.cz/publication/2379097/Generation_of_the_Human_iPSC_Line_from_Spontaneous_Late-Onset_Alzheimer_s_Disease_Patient_with_ApoE3_3_Genotype_and_Sex-__Age-__and_ApoE-Matched_Healthy_Control.pdf

Cite

KADÁKOVÁ, Soňa; Jan RAŠKA; Hana HŘÍBKOVÁ; Veronika FEDOROVÁ; Jiří SEDMÍK; Klára PLEŠINGROVÁ; Kateřina SHEARDOVÁ and Dáša BOHAČIAKOVÁ. Generation of the Human iPSC Line from Spontaneous Late-Onset Alzheimer's Disease Patient with ApoE3/3 Genotype and Sex-, Age-, and ApoE-Matched Healthy Control. STEM CELL RESEARCH. AMSTERDAM: ELSEVIER, 2024, vol. 74, February 2024, p. 1-4. ISSN 1873-5061. Available from: https://dx.doi.org/10.1016/j.scr.2023.103273.

@article{59894,
   author = {Kadáková, Soňa and Raška, Jan and Hříbková, Hana and Fedorová, Veronika and Sedmík, Jiří and Plešingrová, Klára and Sheardová, Kateřina and Bohačiaková, Dáša},
   article_location = {AMSTERDAM},
   article_number = {February 2024},
   doi = {http://dx.doi.org/10.1016/j.scr.2023.103273},
   keywords = {Human iPSC Line; Alzheimer’s Disease; ApoE3/3 Genotype},
   language = {eng},
   issn = {1873-5061},
   journal = {STEM CELL RESEARCH},
   title = {Generation of the Human iPSC Line from Spontaneous Late-Onset Alzheimer's Disease Patient with ApoE3/3 Genotype and Sex-, Age-, and ApoE-Matched Healthy Control},
   url = {https://www.sciencedirect.com/science/article/pii/S1873506123002593?via%3Dihub},
   volume = {74},
   year = {2024}
}

TY  - JOUR
ID  - 59894
AU  - Kadáková, Soňa - Raška, Jan - Hříbková, Hana - Fedorová, Veronika - Sedmík, Jiří - Plešingrová, Klára - Sheardová, Kateřina - Bohačiaková, Dáša
PY  - 2024
TI  - Generation of the Human iPSC Line from Spontaneous Late-Onset Alzheimer's Disease Patient with ApoE3/3 Genotype and Sex-, Age-, and ApoE-Matched Healthy Control
JF  - STEM CELL RESEARCH
VL  - 74
IS  - February 2024
SP  - 1-4
EP  - 1-4
PB  - ELSEVIER
SN  - 1873-5061
KW  - Human iPSC Line
KW  - Alzheimer’s Disease
KW  - ApoE3/3 Genotype
UR  - https://www.sciencedirect.com/science/article/pii/S1873506123002593?via%3Dihub
N2  - Human induced pluripotent stem cell (iPSC) lines were generated from peripheral blood mononuclear cells (PBMCs) isolated from a patient diagnosed with spontaneous late -onset Alzheimer's disease (AD) carrying ApoE3/3 gene and one age-, sex-, and ApoE-matched healthy control. Reprogramming was done using a commercially available Epi5 Reprogramming Kit containing OCT4, SOX2, KLF4, LIN28, and L-MYC as reprogramming factors. The pluripotency of the iPSC lines was verified by the expression of pluripotency markers and by their capacity to differentiate into all three embryonic germ layers in vitro. These newly established iPSC lines offer a valuable platform for in vitro modeling of AD.
ER  -

KADÁKOVÁ, Soňa; Jan RAŠKA; Hana HŘÍBKOVÁ; Veronika FEDOROVÁ; Jiří SEDMÍK; Klára PLEŠINGROVÁ; Kateřina SHEARDOVÁ and Dáša BOHAČIAKOVÁ. Generation of the Human iPSC Line from Spontaneous Late-Onset Alzheimer's Disease Patient with ApoE3/3 Genotype and Sex-, Age-, and ApoE-Matched Healthy Control. \textit{STEM CELL RESEARCH}. AMSTERDAM: ELSEVIER, 2024, vol.~74, February 2024, p.~1-4. ISSN~1873-5061. Available from: https://dx.doi.org/10.1016/j.scr.2023.103273.