FÜZIK, Tibor, Lenka ŠMERDOVÁ, Lucie NEPOVÍMOVÁ, Petra FORMANOVÁ, Petra STRAKOVÁ, Daniel RŮŽEK and Pavel PLEVKA. Asymmetric reconstructions of immature tick-borne encephalitis virus particles reveal defects caused by the assembly process. In NIVB meeting 2023. 2023. ISSN 2336-7210.
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Original name Asymmetric reconstructions of immature tick-borne encephalitis virus particles reveal defects caused by the assembly process
Authors FÜZIK, Tibor, Lenka ŠMERDOVÁ, Lucie NEPOVÍMOVÁ, Petra FORMANOVÁ, Petra STRAKOVÁ, Daniel RŮŽEK and Pavel PLEVKA.
Edition NIVB meeting 2023, 2023.
Other information
Original language English
Type of outcome Konferenční abstrakta
Country of publisher Czech Republic
Confidentiality degree is not subject to a state or trade secret
WWW URL
Organization Středoevropský technologický institut – Repository – Repository
ISSN 2336-7210
Keywords (in Czech) virus; flavirus; klíště; struktura; nezralost; virus klíšťové encefalitidy
Keywords in English virus; flavivirus; tick; structure; nezralý; tick-borne encephalitis virus
Links LX22NPO5103, research and development project.
Changed by Changed by: RNDr. Daniel Jakubík, učo 139797. Changed: 20/1/2024 03:27.
Abstract
Tick-borne encephalitis virus (TBEV) is an enveloped virus belonging to the family Flaviviridae, which causes severe disease of central nervous system in humans. The smooth virion surface is covered by envelope proteins (E-protein), that are together with the membrane proteins (M-protein) anchored in the virus lipid bilayer. During the viral life cycle, the immature non-infectious virus undergoes amaturation process. This process includes proteolytic cleavage of prM and a major reorganization of the envelope proteins on the viral surface.To determine the structure of immature TBEV particles, we purified them from infected tissue culture cells and used cryo-electron microscopy for visualization. The immature particles have “spiky” surface formed by the E-protein-prM-protein complex. We performed single-particle analysis and cryo-electron tomography to reveal the asymmetric nature of the TBEV immature particles. The symmetric, icosahedral, organization of the E-protein-prM-protein spikes on the particle surface is often disrupted by defects introduced during the assembly process of the immature particle. However, these irregularities do not hinder the subsequent maturation process and instead result in mature particles with empty patches in the “herring bone” organization of the mature viral surface.The results provide further insight into the viral maturation process which could be targeted in the future by specific antiviral drugs
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  • a concrete person Mgr. Michal Maňas, uco 2481
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