ZLATOHURSKA, Maryna, Michaela PROCHÁZKOVÁ, Tibor FÜZIK and Pavel PLEVKA. BACTERIOPHAGE PHI812 HEAD ASSEMBLY IN THE CELLS OF STAPHYLOCOCCUS AUREUS. In NIVB Meeting 2023. 2023.
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Basic information
Original name BACTERIOPHAGE PHI812 HEAD ASSEMBLY IN THE CELLS OF STAPHYLOCOCCUS AUREUS
Name in Czech BAKTERIOPHAGE PHI812 HLAVOVÉ USKUPENÍ V BUŇKÁCH STAPHYLOCOCCUS AUREUS
Authors ZLATOHURSKA, Maryna, Michaela PROCHÁZKOVÁ, Tibor FÜZIK and Pavel PLEVKA.
Edition NIVB Meeting 2023, 2023.
Other information
Original language English
Type of outcome Konferenční abstrakta
Country of publisher Czech Republic
Confidentiality degree is not subject to a state or trade secret
WWW URL
Organization Středoevropský technologický institut – Repository – Repository
Keywords (in Czech) bakteriofág; struktura; uspořádání hlav
Keywords in English bacteriophage; structure; head assembly
Links LX22NPO5103, research and development project. 1233487, interní kód Repo.
Changed by Changed by: RNDr. Daniel Jakubík, učo 139797. Changed: 14/12/2023 03:13.
Abstract
Staphylococcus aureus is an opportunistic pathogen causing a diverse range of human illnesses. It possesses a wide potential to develop resistance against antimicrobial agents. Additionally, biofilm-forming strains of S. aureus contribute to the contamination of medical equipment and the establishment of persistent infections. The lytic bacteriophage phi812, a member of the Herelleviridae family, possesses a wide host range, including antibiotics-resistant and biofilm-forming S. aureus strains, which makes it a suitable candidate for the treatment of staphylococcal infections. Nonetheless, it has not been approved for general clinical application because of the insufficient understanding of many aspects of phage biology. To understand the assembly mechanism of phage phi812, we conducted an examination of infected cells using cryo-electron microscopy. We used focused ion beam milling for the preparation of electron-transparent lamellas from infected S. aureus cells and cryo-electron tomography for three-dimensional reconstructions of the cell content, followed by sub-tomogram averaging of the phage assembly intermediates. The phage head assembly starts 15 minutes post-infection at the inner surface of the cytoplasmic membrane. In a short time (30 min) the cell is fully packed with phage assembly intermediates structures, empty, filling, and genome-containing heads and tails connected to fully packed heads. Sub-tomogram averaging of the assembly intermediates revealed distinct classes of phage heads that differ in size and surface features. Our findings offer structural insights into the assembly of the phage phi812 head in near-native conditions.
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  • a concrete person Mgr. Michal Maňas, uco 2481
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