Přehled o publikaci
2023
Improved Screening of Monoclonal Gammopathy Patients by MALDI-TOF Mass Spectrometry
PEČINKA, Lukáš; Monika VLACHOVÁ; Lukáš MORÁŇ; Jana GREGOROVÁ; Volodymyr POROKH et al.Basic information
Original name
Improved Screening of Monoclonal Gammopathy Patients by MALDI-TOF Mass Spectrometry
Authors
PEČINKA, Lukáš; Monika VLACHOVÁ; Lukáš MORÁŇ; Jana GREGOROVÁ; Volodymyr POROKH; Petra KOVAČOVICOVÁ; Martina ALMÁŠI; Luděk POUR; Martin ŠTORK; Josef HAVEL; Sabina ŠEVČÍKOVÁ and Petr VAŇHARA
Edition
Journal of the American Society for Mass Spectrometry, WASHINGTON, American Chemical Society, 2023, 1044-0305
Other information
Language
English
Type of outcome
Article in a journal
Country of publisher
United States of America
Confidentiality degree
is not subject to a state or trade secret
References:
Marked to be transferred to RIV
Yes
RIV identification code
RIV/00216224:14110/23:00132350
Organization
Lékařská fakulta – Repository – Repository
UT WoS
EID Scopus
Keywords in English
MALDI-TOF mass spectrometry; multiple myeloma; plasma cell leukemia; monoclonal gammopathy; principal component analysis; machine learning; partial least-squares-discriminant analysis; molecular profiling; fingerprinting
Links
LX22NPO5102, research and development project. MUNI/A/1298/2022, interní kód Repo. MUNI/A/1301/2022, interní kód Repo. MUNI/A/1370/2022, interní kód Repo. MUNI/A/1575/2023, interní kód Repo. MUNI/11/ACC/3/2022, interní kód Repo. NU21-03-00076, research and development project.
Changed: 6/11/2024 00:50, RNDr. Daniel Jakubík
Abstract
In the original language
gt;50×) of mass spectra obtained from extracts of peripheral blood plasma from monoclonal gammopathy patients. When coupled with advanced statistics and machine learning, the mass spectra profiles enabled the direct identification, classification, and discrimination of multiple myeloma and plasma cell leukemia patients with high accuracy and precision. A model based on PLS-DA achieved the best performance with 71.5% accuracy (95% confidence interval, CI = 57.1–83.3%) when the 10× repeated 5-fold CV was performed. In summary, the two-step extraction protocol improved the analysis of monoclonal gammopathy peripheral blood plasma samples by mass spectrometry and provided a tool for addressing the complex molecular etiology of monoclonal gammopathies.
