Detailed Information on Publication Record

@article{48212,
   author = {Marek, Martin and Chaloupková, Radka and Prudnikova, Tanyana and Sato, Yukari and Rezacova, Pavlina and Nagata, Yuji and Smatanova Kuta, Ivana and Damborský, Jiří},
   article_location = {Amsterdam},
   article_number = {December 2020},
   doi = {http://dx.doi.org/10.1016/j.csbj.2020.05.019},
   keywords = {Haloalkane dehalogenase (HLD); Biocatalysis; Loop-helix transplantation; X-ray crystallography; Enantioselectivity; Access tunnel; Enzyme engineering; Protein design},
   language = {eng},
   issn = {2001-0370},
   journal = {Computational and Structural Biotechnology Journal},
   title = {Structural and catalytic effects of surface loop-helix transplantation within haloalkane dehalogenase family},
   url = {https://www.sciencedirect.com/science/article/pii/S2001037020302828?via%3Dihub},
   volume = {18},
   year = {2020}
}

TY  - JOUR
ID  - 48212
AU  - Marek, Martin - Chaloupková, Radka - Prudnikova, Tanyana - Sato, Yukari - Rezacova, Pavlina - Nagata, Yuji - Smatanova Kuta, Ivana - Damborský, Jiří
PY  - 2020
TI  - Structural and catalytic effects of surface loop-helix transplantation within haloalkane dehalogenase family
JF  - Computational and Structural Biotechnology Journal
VL  - 18
IS  - December 2020
SP  - 1352-1362
EP  - 1352-1362
PB  - Elsevier
SN  - 2001-0370
KW  - Haloalkane dehalogenase (HLD)
KW  - Biocatalysis
KW  - Loop-helix transplantation
KW  - X-ray crystallography
KW  - Enantioselectivity
KW  - Access tunnel
KW  - Enzyme engineering
KW  - Protein design
UR  - https://www.sciencedirect.com/science/article/pii/S2001037020302828?via%3Dihub
N2  - Engineering enzyme catalytic properties is important for basic research as well as for biotechnological applications. We have previously shown that the reshaping of enzyme access tunnels via the deletion of a short surface loop element may yield a haloalkane dehalogenase variant with markedly modified substrate specificity and enantioselectivity. Here, we conversely probed the effects of surface loop-helix transplantation from one enzyme to another within the enzyme family of haloalkane dehalogenases. Precisely, we transplanted a nine-residue long extension of L9 loop and alpha 4 helix from DbjA into the corresponding site of DbeA. Biophysical characterization showed that this fragment transplantation did not affect the overall protein fold or oligomeric state, but lowered protein stability (Delta T-m = -5 to 6 degrees C). Interestingly, the crystal structure of DbeA mutant revealed the unique structural features of enzyme access tunnels, which are known determinants of catalytic properties for this enzyme family. Biochemical data confirmed that insertion increased activity of DbeA with various halogenated substrates and altered its enantioselectivity with several linear beta-bromoalkanes. Our findings support a protein engineering strategy employing surface loop-helix transplantation for construction of novel protein catalysts with modified catalytic properties.
ER  - 

MAREK, Martin, Radka CHALOUPKOVÁ, Tanyana PRUDNIKOVA, Yukari SATO, Pavlina REZACOVA, Yuji NAGATA, Ivana SMATANOVA KUTA and Jiří DAMBORSKÝ. Structural and catalytic effects of surface loop-helix transplantation within haloalkane dehalogenase family. \textit{Computational and Structural Biotechnology Journal}. Amsterdam: Elsevier, 2020, vol.~18, December 2020, p.~1352-1362. ISSN~2001-0370. Available from: https://dx.doi.org/10.1016/j.csbj.2020.05.019.