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@article{48167,
author = {Adamovský, Ondřej and Buerger, Amanda N. and Vespalcová, Hana and Sohag, Shahadur R. and Hanlon, Amy T. and Ginn, Pamela E. and Craft, Serena L. and Smatana, Stanislav and Budinská, Eva and Persico, Maria and Bisesi, Joseph H., Jr. and Martyniuk, Christopher J.},
article_location = {Washington, D.C.},
article_number = {9},
doi = {http://dx.doi.org/10.1021/acs.est.0c00628},
keywords = {DI(2-ETHYLHEXYL) PHTHALATE; AIRWAY INFLAMMATION; INTESTINAL FAILURE; ENTEROCYTE MASS; TIGHT JUNCTIONS; CELLS; NEUROPEPTIDES; ASSOCIATIONS; CITRULLINE; EXPRESSION},
language = {eng},
issn = {0013-936X},
journal = {Technology},
title = {Evaluation of Microbiome-Host Relationships in the Zebrafish Gastrointestinal System Reveals Adaptive Immunity Is a Target of Bis(2-ethylhexyl) Phthalate (DEHP) Exposure},
url = {https://pubs.acs.org/doi/10.1021/acs.est.0c00628},
volume = {54},
year = {2020}
}
TY - JOUR
ID - 48167
AU - Adamovský, Ondřej - Buerger, Amanda N. - Vespalcová, Hana - Sohag, Shahadur R. - Hanlon, Amy T. - Ginn, Pamela E. - Craft, Serena L. - Smatana, Stanislav - Budinská, Eva - Persico, Maria - Bisesi, Joseph H., Jr. - Martyniuk, Christopher J.
PY - 2020
TI - Evaluation of Microbiome-Host Relationships in the Zebrafish Gastrointestinal System Reveals Adaptive Immunity Is a Target of Bis(2-ethylhexyl) Phthalate (DEHP) Exposure
JF - Technology
VL - 54
IS - 9
SP - 5719-5728
EP - 5719-5728
PB - American Chemical Society
SN - 0013-936X
KW - DI(2-ETHYLHEXYL) PHTHALATE
KW - AIRWAY INFLAMMATION
KW - INTESTINAL FAILURE
KW - ENTEROCYTE MASS
KW - TIGHT JUNCTIONS
KW - CELLS
KW - NEUROPEPTIDES
KW - ASSOCIATIONS
KW - CITRULLINE
KW - EXPRESSION
UR - https://pubs.acs.org/doi/10.1021/acs.est.0c00628
N2 - To improve physical characteristics of plastics such as flexibility and durability, producers enrich materials with phthalates such as di-2-(ethylhexyl) phthalate (DEHP). DEHP is a high production volume chemical associated with metabolic and immune disruption in animals and humans. To reveal mechanisms implicated in phthalate-related disruption in the gastrointestinal system, male and female zebrafish were fed DEHP (3 ppm) daily for two months. At the transcriptome level, DEHP significantly upregulated gene networks in the intestine associated with helper T cells' (Th1, Th2, and Th17) specific pathways. The activation of gene networks associated with adaptive immunity was linked to the suppression of networks for tight junction, gap junctional intercellular communication, and transmembrane transporters, all of which are precursors for impaired gut integrity and performance. On a class level, DEHP exposure increased Bacteroidia and Gammaproteobacteria and decreased Verrucomicrobiae in both the male and female gastrointestinal system. Further, in males there was a relative increase in Fusobacteriia and Betaproteobacteria and a relative decrease in Saccharibacteria. Predictive algorithms revealed that the functional shift in the microbiome community, and the metabolites they produce, act to modulate intestinal adaptive immunity. This finding suggests that the gut microbiota may contribute to the adverse effects of DEHP on the host by altering metabolites sensed by both intestinal and immune Th cells. Our results suggest that the microbiome-gut-immune axis can be modified by DEHP and emphasize the value of multiomics approaches to study microbiome-host interactions following chemical perturbations.
ER -
ADAMOVSKÝ, Ondřej, Amanda N. BUERGER, Hana VESPALCOVÁ, Shahadur R. SOHAG, Amy T. HANLON, Pamela E. GINN, Serena L. CRAFT, Stanislav SMATANA, Eva BUDINSKÁ, Maria PERSICO, Joseph H., Jr. BISESI and Christopher J. MARTYNIUK. Evaluation of Microbiome-Host Relationships in the Zebrafish Gastrointestinal System Reveals Adaptive Immunity Is a Target of Bis(2-ethylhexyl) Phthalate (DEHP) Exposure. \textit{Technology}. Washington, D.C.: American Chemical Society, 2020, vol.~54, No~9, p.~5719-5728. ISSN~0013-936X. Available from: https://dx.doi.org/10.1021/acs.est.0c00628.
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