This paper explains the importance of the implementation of gender studies and sexology basics in the initial education of prison staff – mainly the treatment specialists who deal with the prisoners´ sexual deprivation and incidents with a sexual context occurring in the prison setting. To respond to the new staff´s professional needs, the Czech Prison Service Academy teachers must receive sufficient knowledge in this field. The conference organized by the Jan Amos Komenský University in Prague is one of the relevant sources where prison professionals and teachers can acquire deeper insights into the concepts of gender and sexuality.