| HERMANOVA, M., P. HAVRANOVA-VIDLAKOVA, A. ONDRACKOVA, S.S. KUMAR, R. BOWATER a Miroslav FOJTA. Label-free Voltammetric Detection of Products of Terminal Deoxynucleotidyl Transferase Tailing Reaction. Electroanalysis. WEINHEIM: WILEY-VCH Verlag GmbH, 2019, roč. 31, č. 2, s. 246-255. ISSN 1040-0397. |
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@article{33049,
author = {Hermanova, M. and HavranovaandVidlakova, P. and Ondrackova, A. and Kumar, S.S. and Bowater, R. and Fojta, Miroslav},
article_location = {WEINHEIM},
article_number = {2},
keywords = {terminal deoxynucleotidyl transferase; oligonucleotide tailing; DNA electrochemistry; label free; nucleobase; reduction; oxidation},
language = {eng},
issn = {1040-0397},
journal = {Electroanalysis},
title = {Label-free Voltammetric Detection of Products of Terminal Deoxynucleotidyl Transferase Tailing Reaction},
url = {https://onlinelibrary.wiley.com/doi/full/10.1002/elan.201800452},
volume = {31},
year = {2019}
}
TY - JOUR
ID - 33049
AU - Hermanova, M. - Havranova-Vidlakova, P. - Ondrackova, A. - Kumar, S.S. - Bowater, R. - Fojta, Miroslav
PY - 2019
TI - Label-free Voltammetric Detection of Products of Terminal Deoxynucleotidyl Transferase Tailing Reaction
JF - Electroanalysis
VL - 31
IS - 2
SP - 246-255
EP - 246-255
PB - WILEY-VCH Verlag GmbH
SN - 1040-0397
KW - terminal deoxynucleotidyl transferase
KW - oligonucleotide tailing
KW - DNA electrochemistry
KW - label free
KW - nucleobase
KW - reduction
KW - oxidation
UR - https://onlinelibrary.wiley.com/doi/full/10.1002/elan.201800452
N2 - A label-free approach that takes advantage of intrinsic electrochemical activity of nucleobases has been applied to study the products of terminal deoxynucleotidyl transferase (TdT) tailing reaction. DNA homooligonucleotides A(30), C-30 and T-30 were used as primers for the tailing reaction to which a dNTP - or a mixture of dNTPs - and TdT were added to form the tails. Electrochemical detection enabled study of the tailing reaction products created by various combinations of primers and dNTPs, with pyrolytic graphite electrode (PGE) being suitable for remarkably precise analysis of the length of tailing reaction products. Furthermore, the hanging mercury drop electrode (HMDE) was able to reveal formation of various DNA structures, such as DNA hairpins and G-quadruplexes, which influence the behavior of DNA molecules at the negatively charged surface of HMDE. Thus, the described approach proves to be an excellent tool for studying the TdT tailing reactions and for exploring how various DNA structures affect both the tailing reactions and electrochemical behavior of DNA oligonucleotides at electrode surfaces.
ER -
HERMANOVA, M., P. HAVRANOVA-VIDLAKOVA, A. ONDRACKOVA, S.S. KUMAR, R. BOWATER a Miroslav FOJTA. Label-free Voltammetric Detection of Products of Terminal Deoxynucleotidyl Transferase Tailing Reaction. \textit{Electroanalysis}. WEINHEIM: WILEY-VCH Verlag GmbH, 2019, roč.~31, č.~2, s.~246-255. ISSN~1040-0397.
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