J 2017

Directed evolution of biocircuits using conjugative plasmids and CRISPR-Cas9: design and in silico experiments

BENEŠ, David; Alfonso RODRIGUEZ-PATON a Petr SOSÍK

Základní údaje

Originální název

Directed evolution of biocircuits using conjugative plasmids and CRISPR-Cas9: design and in silico experiments

Autoři

BENEŠ, David; Alfonso RODRIGUEZ-PATON a Petr SOSÍK

Vydání

Natural Computing, Springer Netherlands, 2017, 1567-7818

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10201 Computer sciences, information science, bioinformatics

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Označené pro přenos do RIV

Ano

Kód RIV

RIV/47813059:19240/17:A0000085

Organizace

Filozoficko-přírodovědecká fakulta – Slezská univerzita v Opavě – Repozitář

DOI

https://doi.org/10.1007/s11047-016-9595-9

UT WoS

000407824400010

EID Scopus

2-s2.0-85006414967

Klíčová slova anglicky

Biocircuit; Conjugative plasmid; CRISPR-Cas9; Directed evolution; Evolutionary search; Genetic circuit; Programmed evolution

Štítky

SGS132016, ÚI

Příznaky

Mezinárodní význam, Recenzováno

Návaznosti

LQ1602, projekt VaV.
Změněno: 26. 3. 2018 08:50, Mgr. Kamil Matula

Anotace

V originále

Recent links between computer science and synthetic biology allow for construction of many kinds of algorithmic processes within cells, obtained either by a direct engineered design or by an evolutionary search. In the latter case, horizontal gene transfer and especially transfer of plasmids by conjugation is generally respected as a crucial source of genetic diversity in bacteria. While some previous studies focused on mutations as the crucial principle to obtain diversity for engineered evolution, here we consider conjugation itself as a tool to generate diversity from a pre-determined library of biocircuits basic components. The recent development of CRISPR-Cas9 and its programmable DNA cutting ability makes it a powerful selection tool able to remove nonfunctional biocircuits from a cell population. In this paper, we describe a framework for controlled bacterial evolution of biocircuits based on conjugation and on CRISPR-Cas9, resulting in a direct biological implementation of an evolutionary algorithm. In silico experiments provide data to estimate the computational/search capability of plasmid-based engineered evolution.
Zobrazeno: 21. 3. 2026 21:53