Další formáty:
BibTeX
LaTeX
RIS
@article{28376,
author = {Beneš, David and RodriguezandPaton, Alfonso and Sosík, Petr},
article_number = {3},
doi = {http://dx.doi.org/10.1007/s11047-016-9595-9},
keywords = {Biocircuit; Conjugative plasmid; CRISPR-Cas9; Directed evolution; Evolutionary search; Genetic circuit; Programmed evolution},
language = {eng},
issn = {1567-7818},
journal = {Natural Computing},
title = {Directed evolution of biocircuits using conjugative plasmids and CRISPR-Cas9: design and in silico experiments},
url = {https://link.springer.com/article/10.1007%2Fs11047-016-9595-9},
volume = {16},
year = {2017}
}
TY - JOUR
ID - 28376
AU - Beneš, David - Rodriguez-Paton, Alfonso - Sosík, Petr
PY - 2017
TI - Directed evolution of biocircuits using conjugative plasmids and CRISPR-Cas9: design and in silico experiments
JF - Natural Computing
VL - 16
IS - 3
SP - 497-505
EP - 497-505
PB - Springer Netherlands
SN - 1567-7818
KW - Biocircuit
KW - Conjugative plasmid
KW - CRISPR-Cas9
KW - Directed evolution
KW - Evolutionary search
KW - Genetic circuit
KW - Programmed evolution
UR - https://link.springer.com/article/10.1007%2Fs11047-016-9595-9
L2 - https://link.springer.com/article/10.1007%2Fs11047-016-9595-9
N2 - Recent links between computer science and synthetic biology allow for construction of many kinds of algorithmic processes within cells, obtained either by a direct engineered design or by an evolutionary search. In the latter case, horizontal gene transfer and especially transfer of plasmids by conjugation is generally respected as a crucial source of genetic diversity in bacteria. While some previous studies focused on mutations as the crucial principle to obtain diversity for engineered evolution, here we consider conjugation itself as a tool to generate diversity from a pre-determined library of biocircuits basic components. The recent development of CRISPR-Cas9 and its programmable DNA cutting ability makes it a powerful selection tool able to remove nonfunctional biocircuits from a cell population. In this paper, we describe a framework for controlled bacterial evolution of biocircuits based on conjugation and on CRISPR-Cas9, resulting in a direct biological implementation of an evolutionary algorithm. In silico experiments provide data to estimate the computational/search capability of plasmid-based engineered evolution.
ER -
BENEŠ, David, Alfonso RODRIGUEZ-PATON a Petr SOSÍK. Directed evolution of biocircuits using conjugative plasmids and CRISPR-Cas9: design and in silico experiments. \textit{Natural Computing}. Springer Netherlands, roč.~16, č.~3, s.~497-505. ISSN~1567-7818. doi:10.1007/s11047-016-9595-9. 2017.
|
