Detailed Information on Publication Record
2016
Atomic force microscopy combined with human pluripotent stem cell derived cardiomyocytes for biomechanical sensing
PEŠL, Martin, Jan PŘIBYL, Ivana AĆIMOVIĆ, Aleksandra VILOTIĆ, Šárka JELÍNKOVÁ et. al.Basic information
Original name
Atomic force microscopy combined with human pluripotent stem cell derived cardiomyocytes for biomechanical sensing
Authors
PEŠL, Martin (203 Czech Republic, belonging to the institution), Jan PŘIBYL (203 Czech Republic, belonging to the institution), Ivana AĆIMOVIĆ (688 Serbia, belonging to the institution), Aleksandra VILOTIĆ (688 Serbia, belonging to the institution), Šárka JELÍNKOVÁ (203 Czech Republic, belonging to the institution), Anton SALYKIN (643 Russian Federation, belonging to the institution), Alain LACAMPAGNE (250 France), Petr DVOŘÁK (203 Czech Republic, belonging to the institution), Albano MELI (250 France, belonging to the institution), Petr SKLÁDAL (203 Czech Republic, belonging to the institution) and Vladimír ROTREKL (203 Czech Republic, guarantor, belonging to the institution)
Edition
bioelectronics : the international journal for the professional involved with research, technology and applications of biosensors and related devices, Oxford, Elsevier Science, 2016, 0956-5663
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
Biotechnology and bionics
Country of publisher
United Kingdom of Great Britain and Northern Ireland
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
RIV identification code
RIV/00216224:14110/16:00087965
Organization
Lékařská fakulta – Repository – Repository
UT WoS
000382410100100
Keywords in English
Micromechanical biosensor; Human stem cell; Cardiomyocyte contraction; Drug testing
Links
ED1.1.00/02.0068, research and development project. GA13-19910S, research and development project. GBP302/12/G157, research and development project. LQ1601, research and development project. 2SGA2744, interní kód Repo.
Změněno: 3/9/2020 01:38, RNDr. Daniel Jakubík
Abstract
V originále
Cardiomyocyte contraction and relaxation are important parameters of cardiac function altered in many heart pathologies. Biosensing of these parameters represents an important tool in drug development and disease modeling. Human embryonic stem cells and especially patient specific induced pluripotent stem cell-derived cardiomyocytes are well established as cardiac disease model.. Here, a live stem cell derived embryoid body (EB) based cardiac cell syncytium served as a biorecognition element coupled to the microcantilever probe from atomic force microscope thus providing reliable micromechanical cellular biosensor suitable for whole-day testing. The biosensor was optimized regarding the type of cantilever, temperature and exchange of media; in combination with standardized protocol, it allowed testing of compounds and conditions affecting the biomechanical properties of EB. The studied effectors included calcium , drugs modulating the catecholaminergic fight-or-flight stress response such as the beta-adrenergic blocker metoprolol and the beta-adrenergic agonist isoproterenol. Arrhythmogenic effects were studied using caffeine. Furthermore, with EBs originating from patient's stem cells, this biosensor can help to characterize heart diseases such as dystrophies.