Atomic force microscopy combined with human pluripotent stem
cell derived cardiomyocytes for biomechanical sensing

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Atomic force microscopy combined with human pluripotent stem cell derived cardiomyocytes for ...

Podrobný výpis o publikaci

J 2016

Atomic force microscopy combined with human pluripotent stem cell derived cardiomyocytes for biomechanical sensing

PEŠL, Martin, Jan PŘIBYL, Ivana AĆIMOVIĆ, Aleksandra VILOTIĆ, Šárka JELÍNKOVÁ et. al.

Základní údaje

Originální název

Atomic force microscopy combined with human pluripotent stem cell derived cardiomyocytes for biomechanical sensing

Autoři

PEŠL, Martin (203 Česká republika, domácí), Jan PŘIBYL (203 Česká republika, domácí), Ivana AĆIMOVIĆ (688 Srbsko, domácí), Aleksandra VILOTIĆ (688 Srbsko, domácí), Šárka JELÍNKOVÁ (203 Česká republika, domácí), Anton SALYKIN (643 Rusko, domácí), Alain LACAMPAGNE (250 Francie), Petr DVOŘÁK (203 Česká republika, domácí), Albano MELI (250 Francie, domácí), Petr SKLÁDAL (203 Česká republika, domácí) a Vladimír ROTREKL (203 Česká republika, garant, domácí)

Vydání

bioelectronics : the international journal for the professional involved with research, technology and applications of biosensors and related devices, Oxford, Elsevier Science, 2016, 0956-5663

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

Biotechnologie a bionika

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Kód RIV

RIV/00216224:14110/16:00087965

Organizace

Lékařská fakulta – Masarykova univerzita – Repozitář

UT WoS

000382410100100

Klíčová slova anglicky

Micromechanical biosensor; Human stem cell; Cardiomyocyte contraction; Drug testing

Návaznosti

ED1.1.00/02.0068, projekt VaV. GA13-19910S, projekt VaV. GBP302/12/G157, projekt VaV. LQ1601, projekt VaV. 2SGA2744, interní kód Repo.

Změněno: 3. 9. 2020 01:38, RNDr. Daniel Jakubík

Anotace

V originále

Cardiomyocyte contraction and relaxation are important parameters of cardiac function altered in many heart pathologies. Biosensing of these parameters represents an important tool in drug development and disease modeling. Human embryonic stem cells and especially patient specific induced pluripotent stem cell-derived cardiomyocytes are well established as cardiac disease model.. Here, a live stem cell derived embryoid body (EB) based cardiac cell syncytium served as a biorecognition element coupled to the microcantilever probe from atomic force microscope thus providing reliable micromechanical cellular biosensor suitable for whole-day testing. The biosensor was optimized regarding the type of cantilever, temperature and exchange of media; in combination with standardized protocol, it allowed testing of compounds and conditions affecting the biomechanical properties of EB. The studied effectors included calcium , drugs modulating the catecholaminergic fight-or-flight stress response such as the beta-adrenergic blocker metoprolol and the beta-adrenergic agonist isoproterenol. Arrhythmogenic effects were studied using caffeine. Furthermore, with EBs originating from patient's stem cells, this biosensor can help to characterize heart diseases such as dystrophies.

Přiložené soubory

http://is.muni.cz/repo/1347024/2016_AFM_CMs.pdf

Požádat o autorskou verzi souboru

http://is.muni.cz/repo/1347024/EL_1347024.pdf

Požádat o autorskou verzi souboru

https://is.muni.cz/publication/1347024/2016_AFM_CMs.pdf

Požádat o autorskou verzi souboru

https://is.muni.cz/publication/1347024/EL_1347024.pdf

Požádat o autorskou verzi souboru

Citovat

PEŠL, Martin, Jan PŘIBYL, Ivana AĆIMOVIĆ, Aleksandra VILOTIĆ, Šárka JELÍNKOVÁ, Anton SALYKIN, Alain LACAMPAGNE, Petr DVOŘÁK, Albano MELI, Petr SKLÁDAL a Vladimír ROTREKL. Atomic force microscopy combined with human pluripotent stem cell derived cardiomyocytes for biomechanical sensing. bioelectronics : the international journal for the professional involved with research, technology and applications of biosensors and related devices. Oxford: Elsevier Science, 2016, roč. 85, "neuvedeno", s. 751-757. ISSN 0956-5663.

@article{22131,
   author = {Pešl, Martin and Přibyl, Jan and Aćimović, Ivana and Vilotić, Aleksandra and Jelínková, Šárka and Salykin, Anton and Lacampagne, Alain and Dvořák, Petr and Meli, Albano and Skládal, Petr and Rotrekl, Vladimír},
   article_location = {Oxford},
   article_number = {"neuvedeno"},
   keywords = {Micromechanical biosensor; Human stem cell; Cardiomyocyte contraction; Drug testing},
   language = {eng},
   issn = {0956-5663},
   journal = {bioelectronics : the international journal for the professional involved with research, technology and applications of biosensors and related devices},
   title = {Atomic force microscopy combined with human pluripotent stem cell derived cardiomyocytes for biomechanical sensing},
   url = {http://www.sciencedirect.com/science/article/pii/S0956566316305036},
   volume = {85},
   year = {2016}
}

TY  - JOUR
ID  - 22131
AU  - Pešl, Martin - Přibyl, Jan - Aćimović, Ivana - Vilotić, Aleksandra - Jelínková, Šárka - Salykin, Anton - Lacampagne, Alain - Dvořák, Petr - Meli, Albano - Skládal, Petr - Rotrekl, Vladimír
PY  - 2016
TI  - Atomic force microscopy combined with human pluripotent stem cell derived cardiomyocytes for biomechanical sensing
JF  - bioelectronics : the international journal for the professional involved with research, technology and applications of biosensors and related devices
VL  - 85
IS  - "neuvedeno"
SP  - 751-757
EP  - 751-757
PB  - Elsevier Science
SN  - 0956-5663
KW  - Micromechanical biosensor
KW  - Human stem cell
KW  - Cardiomyocyte contraction
KW  - Drug testing
UR  - http://www.sciencedirect.com/science/article/pii/S0956566316305036
N2  - Cardiomyocyte contraction and relaxation are important parameters of cardiac function altered in many heart pathologies. Biosensing of these parameters represents an important tool in drug development and disease modeling. Human embryonic stem cells and especially patient specific induced pluripotent stem cell-derived cardiomyocytes are well established as cardiac disease model.. Here, a live stem cell derived embryoid body (EB) based cardiac cell syncytium served as a biorecognition element coupled to the microcantilever probe from atomic force microscope thus providing reliable micromechanical cellular biosensor suitable for whole-day testing. The biosensor was optimized regarding the type of cantilever, temperature and exchange of media; in combination with standardized protocol, it allowed testing of compounds and conditions affecting the biomechanical properties of EB. The studied effectors included calcium , drugs modulating the catecholaminergic fight-or-flight stress response such as the beta-adrenergic blocker metoprolol and the beta-adrenergic agonist isoproterenol. Arrhythmogenic effects were studied using caffeine. Furthermore, with EBs originating from patient's stem cells, this biosensor can help to characterize heart diseases such as dystrophies.
ER  -

PEŠL, Martin, Jan PŘIBYL, Ivana A$\backslash$'CIMOVI$\backslash$'C, Aleksandra VILOTI$\backslash$'C, Šárka JELÍNKOVÁ, Anton SALYKIN, Alain LACAMPAGNE, Petr DVOŘÁK, Albano MELI, Petr SKLÁDAL a Vladimír ROTREKL. Atomic force microscopy combined with human pluripotent stem cell derived cardiomyocytes for biomechanical sensing. \textit{bioelectronics : the international journal for the professional involved with research, technology and applications of biosensors and related devices}. Oxford: Elsevier Science, 2016, roč.~85, ''neuvedeno'', s.~751-757. ISSN~0956-5663.