Purpose: The main goal of this paper is to argue E-learning can be a viable alternative teaching method for Information Literacy according to a comparation of librarian’s time spent face-to-face teaching with tutoring the E-learning course, average time spent a week on learning by the students, time flexibility of E-learning, students’ satisfaction with E-learning and students’ ability to gain practical skills and theoretical knowledge through E-learning. Design/methodology/approach: Satisfaction of medical students with E-learning and their average weekly time spent learning was assessed through surveys designed in Google Documents. Weekly time spent by students learning in class and the number of librarian teaching hours were set by the university schedule and time spent on tutoring E-learning was measured. Details of accesses to study materials and submission of tasks as well as exam results were collected from Masaryk University Learning Management System. Findings: In 2011 50% less time was expended on tutoring E-learning than time spent with the same number of students in the previous three years in the classroom. One third of the students learned for more hours a week with E-learning than students in class. No significant difference in gained theoretical knowledge between these students was found. On average 90% of tasks submitted to E-learning were correct the first time. E-learning was appreciated by the students for its time (93%) and space (83 %) flexibility, the online materials (62%) and self-managing learning time (55%). Details of access to the study materials confirmed time flexibility. Originality/value: Due to time saved and considering the lack of any significant difference in the knowledge gained by students, E-learning can be a viable alternative teaching method for Information Literacy.