LATTOVÁ, Erika, Jana SKŘIČKOVÁ and Zbyněk ZDRÁHAL. Applicability of Phenylhydrazine Labeling for Structural Studies of Fucosylated N-Glycans. Anal. Chem. American Chemical Society, vol. 91, No 13, p. 7985-7990. ISSN 0003-2700. 2019.
Other formats:   BibTeX LaTeX RIS
Basic information
Original name Applicability of Phenylhydrazine Labeling for Structural Studies of Fucosylated N-Glycans
Authors LATTOVÁ, Erika (703 Slovakia, guarantor, belonging to the institution), Jana SKŘIČKOVÁ (203 Czech Republic, belonging to the institution) and Zbyněk ZDRÁHAL (203 Czech Republic, belonging to the institution).
Edition Anal. Chem. American Chemical Society, 2019, 0003-2700.
Other information
Original language English
Type of outcome Article in a journal
Country of publisher United States of America
Confidentiality degree is not subject to a state or trade secret
WWW URL
RIV identification code RIV/00216224:14740/19:00110252
Organization Středoevropský technologický institut – Repository – Repository
UT WoS 000474477900004
Keywords in English TANDEM MASS-SPECTROMETRY; DECAY FRAGMENTATION; PROTEIN; GLYCOSYLATION; FUCOSE; CORE
Links EF17_050/0008496, research and development project. LM2015043, research and development project.
Changed by Changed by: RNDr. Daniel Jakubík, učo 139797. Changed: 8/9/2020 19:51.
Abstract
Fucosylation is a common modification, and its site in glycans refers to different normal and pathological processes. Despite intensive research, there is still a lack of methods to discriminate unambiguously the fucose position in one-step. In this work, we propose utility of phenylhydrazine (PHN) labeling for structural studies of fucosylated N-glycans by tandem MALDI mass spectrometry (MS) in the positive ion mode. PHN-tag influences the production of specific ion types, and the MS/MS fragmentation pattern provides useful structural information. All types of core fucosylated N-glycans have produced two abundant ions consistent with B- and C-glycosidic cleavages corresponding to the loss of the FucGlcNAcPHN residue with a mass 457 and 441 Da from the parent ions. These types of fragment ions in N-glycans without a core fucose were associated with the loss of the GlcNAcPHN unit (311 and 295 Da), and fucose cleavage followed the loss of the chitobiose residue. Since diagnostic useful cleavages produce peaks with significant intensities, this approach is also beneficial for rapid recognition of antenna from core fucosylation in glycans detected with low abundances. Moreover, in multifucosylated glycans, this type of labeling allows to distinguish how many fucose residues are on the specific antenna and provides additional information on the topology of N-glycans, such as type of antennarity or identification of bisecting moiety. The practical applicability of the approach is demonstrated on the analysis of multifucosylated N-glycans detected with lower abundances in lung cancer samples.
Type Name Uploaded/Created by Uploaded/Created Rights
Accepted_acs.analchem.9b01321.pdf   File version 10/8/2019

Properties

Name
Accepted_acs.analchem.9b01321.pdf
Address within IS
https://repozitar.cz/auth/repo/36047/690180/
Address for the users outside IS
https://repozitar.cz/repo/36047/690180/
Address within Manager
https://repozitar.cz/auth/repo/36047/690180/?info
Address within Manager for the users outside IS
https://repozitar.cz/repo/36047/690180/?info
Uploaded/Created
Sat 10/8/2019 00:57

Rights

Right to read
  • anyone on the Internet
Right to upload
 
Right to administer:
  • a concrete person Mgr. Lucie Vařechová, uco 106253
  • a concrete person RNDr. Daniel Jakubík, uco 139797
  • a concrete person Mgr. Jolana Surýnková, uco 220973
Attributes
 
Accepted_acs.analchem.9b01321.pdf   File version 15/8/2020

Properties

Name
Accepted_acs.analchem.9b01321.pdf
Address within IS
https://repozitar.cz/auth/repo/36047/872322/
Address for the users outside IS
https://repozitar.cz/repo/36047/872322/
Address within Manager
https://repozitar.cz/auth/repo/36047/872322/?info
Address within Manager for the users outside IS
https://repozitar.cz/repo/36047/872322/?info
Uploaded/Created
Sat 15/8/2020 05:30

Rights

Right to read
  • anyone on the Internet
Right to upload
 
Right to administer:
  • a concrete person Mgr. Lucie Vařechová, uco 106253
  • a concrete person RNDr. Daniel Jakubík, uco 139797
  • a concrete person Mgr. Jolana Surýnková, uco 220973
Attributes
 
Print
Add to clipboard Displayed: 29/3/2024 06:59