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@article{36047, author = {Lattová, Erika and Skřičková, Jana and Zdráhal, Zbyněk}, article_number = {13}, keywords = {TANDEM MASS-SPECTROMETRY; DECAY FRAGMENTATION; PROTEIN; GLYCOSYLATION; FUCOSE; CORE}, language = {eng}, issn = {0003-2700}, journal = {Anal. Chem.}, title = {Applicability of Phenylhydrazine Labeling for Structural Studies of Fucosylated N-Glycans}, url = {https://pubs.acs.org/doi/10.1021/acs.analchem.9b01321}, volume = {91}, year = {2019} }
TY - JOUR ID - 36047 AU - Lattová, Erika - Skřičková, Jana - Zdráhal, Zbyněk PY - 2019 TI - Applicability of Phenylhydrazine Labeling for Structural Studies of Fucosylated N-Glycans JF - Anal. Chem. VL - 91 IS - 13 SP - 7985-7990 EP - 7985-7990 PB - American Chemical Society SN - 0003-2700 KW - TANDEM MASS-SPECTROMETRY KW - DECAY FRAGMENTATION KW - PROTEIN KW - GLYCOSYLATION KW - FUCOSE KW - CORE UR - https://pubs.acs.org/doi/10.1021/acs.analchem.9b01321 N2 - Fucosylation is a common modification, and its site in glycans refers to different normal and pathological processes. Despite intensive research, there is still a lack of methods to discriminate unambiguously the fucose position in one-step. In this work, we propose utility of phenylhydrazine (PHN) labeling for structural studies of fucosylated N-glycans by tandem MALDI mass spectrometry (MS) in the positive ion mode. PHN-tag influences the production of specific ion types, and the MS/MS fragmentation pattern provides useful structural information. All types of core fucosylated N-glycans have produced two abundant ions consistent with B- and C-glycosidic cleavages corresponding to the loss of the FucGlcNAcPHN residue with a mass 457 and 441 Da from the parent ions. These types of fragment ions in N-glycans without a core fucose were associated with the loss of the GlcNAcPHN unit (311 and 295 Da), and fucose cleavage followed the loss of the chitobiose residue. Since diagnostic useful cleavages produce peaks with significant intensities, this approach is also beneficial for rapid recognition of antenna from core fucosylation in glycans detected with low abundances. Moreover, in multifucosylated glycans, this type of labeling allows to distinguish how many fucose residues are on the specific antenna and provides additional information on the topology of N-glycans, such as type of antennarity or identification of bisecting moiety. The practical applicability of the approach is demonstrated on the analysis of multifucosylated N-glycans detected with lower abundances in lung cancer samples. ER -
LATTOVÁ, Erika, Jana SKŘIČKOVÁ and Zbyněk ZDRÁHAL. Applicability of Phenylhydrazine Labeling for Structural Studies of Fucosylated N-Glycans. \textit{Anal. Chem.}. American Chemical Society, 2019, vol.~91, No~13, p.~7985-7990. ISSN~0003-2700.
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