HERMANOVA, M., P. HAVRANOVA-VIDLAKOVA, A. ONDRACKOVA, S.S. KUMAR, R. BOWATER and Miroslav FOJTA. Label-free Voltammetric Detection of Products of Terminal Deoxynucleotidyl Transferase Tailing Reaction. Electroanalysis. WEINHEIM: WILEY-VCH Verlag GmbH, 2019, vol. 31, No 2, p. 246-255. ISSN 1040-0397. |
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@article{33049, author = {Hermanova, M. and HavranovaandVidlakova, P. and Ondrackova, A. and Kumar, S.S. and Bowater, R. and Fojta, Miroslav}, article_location = {WEINHEIM}, article_number = {2}, keywords = {terminal deoxynucleotidyl transferase; oligonucleotide tailing; DNA electrochemistry; label free; nucleobase; reduction; oxidation}, language = {eng}, issn = {1040-0397}, journal = {Electroanalysis}, title = {Label-free Voltammetric Detection of Products of Terminal Deoxynucleotidyl Transferase Tailing Reaction}, url = {https://onlinelibrary.wiley.com/doi/full/10.1002/elan.201800452}, volume = {31}, year = {2019} }
TY - JOUR ID - 33049 AU - Hermanova, M. - Havranova-Vidlakova, P. - Ondrackova, A. - Kumar, S.S. - Bowater, R. - Fojta, Miroslav PY - 2019 TI - Label-free Voltammetric Detection of Products of Terminal Deoxynucleotidyl Transferase Tailing Reaction JF - Electroanalysis VL - 31 IS - 2 SP - 246-255 EP - 246-255 PB - WILEY-VCH Verlag GmbH SN - 1040-0397 KW - terminal deoxynucleotidyl transferase KW - oligonucleotide tailing KW - DNA electrochemistry KW - label free KW - nucleobase KW - reduction KW - oxidation UR - https://onlinelibrary.wiley.com/doi/full/10.1002/elan.201800452 N2 - A label-free approach that takes advantage of intrinsic electrochemical activity of nucleobases has been applied to study the products of terminal deoxynucleotidyl transferase (TdT) tailing reaction. DNA homooligonucleotides A(30), C-30 and T-30 were used as primers for the tailing reaction to which a dNTP - or a mixture of dNTPs - and TdT were added to form the tails. Electrochemical detection enabled study of the tailing reaction products created by various combinations of primers and dNTPs, with pyrolytic graphite electrode (PGE) being suitable for remarkably precise analysis of the length of tailing reaction products. Furthermore, the hanging mercury drop electrode (HMDE) was able to reveal formation of various DNA structures, such as DNA hairpins and G-quadruplexes, which influence the behavior of DNA molecules at the negatively charged surface of HMDE. Thus, the described approach proves to be an excellent tool for studying the TdT tailing reactions and for exploring how various DNA structures affect both the tailing reactions and electrochemical behavior of DNA oligonucleotides at electrode surfaces. ER -
HERMANOVA, M., P. HAVRANOVA-VIDLAKOVA, A. ONDRACKOVA, S.S. KUMAR, R. BOWATER and Miroslav FOJTA. Label-free Voltammetric Detection of Products of Terminal Deoxynucleotidyl Transferase Tailing Reaction. \textit{Electroanalysis}. WEINHEIM: WILEY-VCH Verlag GmbH, 2019, vol.~31, No~2, p.~246-255. ISSN~1040-0397.
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