gt;20% mitoses) but repeatedly failed to detect specific rearrangements, such as dicentric chromosomes and derived chromosomes involving more than two chromosomes. For this purpose, the analysis against the T2T reference would be beneficial. Overall, we uncovered significantly greater SV complexity in all ten cases by combining short-read WGS, ONT, and OGM than identified by low-throughput methods. These pilot data will serve to adjust the analytical approaches for further larger patient cohorts.