Assessment of the Cronobacter sakazakii risk in reconstituted
infant formula

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Assessment of the Cronobacter sakazakii risk in reconstituted infant formula

Přehled o publikaci

J 2025

Assessment of the Cronobacter sakazakii risk in reconstituted infant formula

BURSOVA, Sarka; Danka HARUŠTIAKOVÁ; Lenka NECIDOVA; Iveta VANKOVA; Veronika CURECKOVA et. al.

Basic information

Original name

Assessment of the Cronobacter sakazakii risk in reconstituted infant formula

Authors

BURSOVA, Sarka; Danka HARUŠTIAKOVÁ; Lenka NECIDOVA; Iveta VANKOVA; Veronika CURECKOVA; Matej TKAC; Katerina STOJANOVA; Radka HULANKOVA and Jozef GOLIAN

Edition

Acta veterinaria (Brno), Brno, University of Veterinary and Pharmaceutical Sciences Brno, 2025, 0001-7213

Other information

Language

English

Type of outcome

Article in a journal

Country of publisher

Czech Republic

Confidentiality degree

is not subject to a state or trade secret

References:

URL

Organization

Přírodovědecká fakulta – Repository – Repository

DOI

http://dx.doi.org/10.2754/avb202594010067

UT WoS

001460138200009

EID Scopus

2-s2.0-105001805905

Keywords in English

Predictive microbiology; Baranyi-Roberts model; lag phase; growth rate

Changed: 18/7/2025 00:49, RNDr. Daniel Jakubík

Abstract

V originále

The study focused on assessing Cronobacter sakazakii growth in reconstituted powdered infant formula at temperatures ranging from 5 degrees C to 48 degrees C using the Baranyi-Roberts model. The count of C. sakazakii was determined by the plate method on ESIA agar (44 degrees C, 24 h). Bacteria grew in reconstituted milk only at temperatures above 8 degrees C. The lag phase duration decreased with increasing temperature, from approximately 123.0-141.0 h at 8 degrees C to 0.931-1.281 h at 44 degrees C. The growth rate ranged from 0.025-0.027 ln cfu/ml/h (8 degrees C) to 2.441-2.633 ln cfu/ml/h (44 degrees C). The resulting growth models imply an increase of more than 4 orders of magnitude in the number of C. sakazakii in less than 17 h at 24 degrees C; at temperatures of 27 degrees C and above, the bacteria reach the critical concentration considered in our study (8 log cfu) in a few hours (4.5-11.9 h). In conclusion, it is unsafe and inappropriate to store reconstituted infant milk at temperatures higher than 8 degrees C.

Files attached

https://is.muni.cz/publication/2507599/2507599.pdf

Cite

BURSOVA, Sarka; Danka HARUŠTIAKOVÁ; Lenka NECIDOVA; Iveta VANKOVA; Veronika CURECKOVA; Matej TKAC; Katerina STOJANOVA; Radka HULANKOVA and Jozef GOLIAN. Assessment of the Cronobacter sakazakii risk in reconstituted infant formula. Acta veterinaria (Brno). Brno: University of Veterinary and Pharmaceutical Sciences Brno, 2025, vol. 94, No 1, p. 67-75. ISSN 0001-7213. Available from: https://dx.doi.org/10.2754/avb202594010067.

@article{77539,
   author = {Bursova, Sarka and Haruštiaková, Danka and Necidova, Lenka and Vankova, Iveta and Cureckova, Veronika and Tkac, Matej and Stojanova, Katerina and Hulankova, Radka and Golian, Jozef},
   article_location = {Brno},
   article_number = {1},
   doi = {http://dx.doi.org/10.2754/avb202594010067},
   keywords = {Predictive microbiology; Baranyi-Roberts model; lag phase; growth rate},
   language = {eng},
   issn = {0001-7213},
   journal = {Acta veterinaria (Brno)},
   title = {Assessment of the Cronobacter sakazakii risk in reconstituted infant formula},
   url = {https://actavet.vfu.cz/94/1/0067/},
   volume = {94},
   year = {2025}
}

TY  - JOUR
ID  - 77539
AU  - Bursova, Sarka - Haruštiaková, Danka - Necidova, Lenka - Vankova, Iveta - Cureckova, Veronika - Tkac, Matej - Stojanova, Katerina - Hulankova, Radka - Golian, Jozef
PY  - 2025
TI  - Assessment of the Cronobacter sakazakii risk in reconstituted infant formula
JF  - Acta veterinaria (Brno)
VL  - 94
IS  - 1
SP  - 67-75
EP  - 67-75
PB  - University of Veterinary and Pharmaceutical Sciences Brno
SN  - 0001-7213
KW  - Predictive microbiology
KW  - Baranyi-Roberts model
KW  - lag phase
KW  - growth rate
UR  - https://actavet.vfu.cz/94/1/0067/
N2  - The study focused on assessing Cronobacter sakazakii growth in reconstituted powdered infant formula at temperatures ranging from 5 degrees C to 48 degrees C using the Baranyi-Roberts model. The count of C. sakazakii was determined by the plate method on ESIA agar (44 degrees C, 24 h). Bacteria grew in reconstituted milk only at temperatures above 8 degrees C. The lag phase duration decreased with increasing temperature, from approximately 123.0-141.0 h at 8 degrees C to 0.931-1.281 h at 44 degrees C. The growth rate ranged from 0.025-0.027 ln cfu/ml/h (8 degrees C) to 2.441-2.633 ln cfu/ml/h (44 degrees C). The resulting growth models imply an increase of more than 4 orders of magnitude in the number of C. sakazakii in less than 17 h at 24 degrees C; at temperatures of 27 degrees C and above, the bacteria reach the critical concentration considered in our study (8 log cfu) in a few hours (4.5-11.9 h). In conclusion, it is unsafe and inappropriate to store reconstituted infant milk at temperatures higher than 8 degrees C.
ER  -

BURSOVA, Sarka; Danka HARUŠTIAKOVÁ; Lenka NECIDOVA; Iveta VANKOVA; Veronika CURECKOVA; Matej TKAC; Katerina STOJANOVA; Radka HULANKOVA and Jozef GOLIAN. Assessment of the Cronobacter sakazakii risk in reconstituted infant formula. \textit{Acta veterinaria (Brno)}. Brno: University of Veterinary and Pharmaceutical Sciences Brno, 2025, vol.~94, No~1, p.~67-75. ISSN~0001-7213. Available from: https://dx.doi.org/10.2754/avb202594010067.