Přehled o publikaci
2025
Reliable detection of CNS lymphoma-derived circulating tumor DNA in cerebrospinal fluid using multi-biomarker NGS profiling: insights from a real-world study
NAVRKALOVÁ, Veronika, Andrea MAREČKOVÁ, Samuel HRICKO, Viera HRABČÁKOVÁ, Lenka RADOVÁ et. al.Basic information
Original name
Reliable detection of CNS lymphoma-derived circulating tumor DNA in cerebrospinal fluid using multi-biomarker NGS profiling: insights from a real-world study
Authors
NAVRKALOVÁ, Veronika, Andrea MAREČKOVÁ, Samuel HRICKO, Viera HRABČÁKOVÁ, Lenka RADOVÁ, Václav KUBEŠ, Jakub Paweł PORC, Tomáš REIGL, Šárka POSPÍŠILOVÁ, Jana KOTAŠKOVÁ and Andrea JANÍKOVÁ
Edition
BIOMARKER RESEARCH, LONDON, BMC, 2025, 2050-7771
Other information
Language
English
Type of outcome
Article in a journal
Country of publisher
United Kingdom of Great Britain and Northern Ireland
Confidentiality degree
is not subject to a state or trade secret
References:
Organization
Lékařská fakulta – Repository – Repository
UT WoS
001485606600001
EID Scopus
2-s2.0-105004890394
Keywords in English
ctDNA detection; Liquid biopsy; Multi-target NGS; Clinical practice; CNS lymphomas
Links
LM2018132, research and development project. LM2023067, research and development project. LX22NPO5102, research and development project. LX22NPO5107, research and development project. MUNI/A/1685/2024, interní kód Repo. NU22-08-00227, research and development project. e-INFRA CZ II, large research infrastructures.
Changed: 30/5/2025 00:50, RNDr. Daniel Jakubík
Abstract
V originále
lt; 0.001). Parallel analysis of tumor DNA in both cell-free and cellular DNA from CSF makes the probability of primary or secondary CNS malignancy detection even higher. Conclusions Our prospective, tissue-agnostic approach highlights the feasibility of ctDNA sequencing by a commonplace and affordable method, offering higher sensitivity to detect CNS infiltration with lymphoma than standard cell-analyzing techniques. We accentuate the benefit of a multi-target NGS approach and adequate CSF sampling to obtain satisfactory diagnostic yield. Less invasive liquid biopsy testing by comprehensive NGS complements standard procedures in the diagnostics and management of CNSL patients, especially when encountering limitations.
