MoaB2, a newly identified transcription factor, binds to σA in
Mycobacterium smegmatis

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MoaB2, a newly identified transcription factor, binds to σA in Mycobacterium smegmatis

Přehled o publikaci

J 2024

MoaB2, a newly identified transcription factor, binds to σA in Mycobacterium smegmatis

BREZOVSKA, Barbora; Subhash NARASIMHAN; Michaela SIKOVA; Hana SANDEROVA; Tomas KOVAL et. al.

Základní údaje

Originální název

MoaB2, a newly identified transcription factor, binds to σA in Mycobacterium smegmatis

Autoři

BREZOVSKA, Barbora; Subhash NARASIMHAN (356 Indie, domácí); Michaela SIKOVA; Hana SANDEROVA; Tomas KOVAL; Nabajyoti BORAH; Mahmoud SHOMAN; Debora POSPISILOVA; Viola Vankova HAUSNEROVA; Dávid TUŽINČIN (703 Slovensko, domácí); Martin ČERNÝ (203 Česká republika, domácí); Jan KOMÁREK (203 Česká republika, domácí); Martina JANOUSKOVA; Milada KAMBOVA; Petr HALADA; Alena KRENKOVA; Martin HUBALEK; Maria TRUNDOVA; Jan DOHNALEK; Jarmila HNILICOVA; Lukáš ŽÍDEK (203 Česká republika, domácí) a Libor KRASNY

Vydání

Journal of Bacteriology, WASHINGTON, AMER SOC MICROBIOLOGY, 2024, 0021-9193

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Kód RIV

RIV/00216224:14740/24:00138935

Organizace

Středoevropský technologický institut – Masarykova univerzita – Repozitář

DOI

http://dx.doi.org/10.1128/jb.00066-24

UT WoS

001348035500001

EID Scopus

2-s2.0-85213063372

Klíčová slova anglicky

MoaB2; sigma(A); mycobacteria; RNA polymerase; transcription

Návaznosti

EF18_046/0015974, projekt VaV. GA19-12956S, projekt VaV. GA22-12023S, projekt VaV. LM2018127, projekt VaV. LM2018131, projekt VaV. LX22NPO5103, projekt VaV. CIISB III, velká výzkumná infrastruktura.

Změněno: 4. 6. 2025 00:50, RNDr. Daniel Jakubík

Anotace

V originále

In mycobacteria, σA is the primary sigma factor. This essential protein binds to RNA polymerase (RNAP) and mediates transcription initiation of housekeeping genes. Our knowledge about this factor in mycobacteria is limited. Here, we performed an unbiased search for interacting partners of Mycobacterium smegmatis σA. The search revealed a number of proteins; prominent among them was MoaB2. The σA-MoaB2 interaction was validated and characterized by several approaches, revealing that it likely does not require RNAP and is specific, as alternative σ factors (e.g., closely related σB) do not interact with MoaB2. The structure of MoaB2 was solved by X-ray crystallography. By immunoprecipitation and nuclear magnetic resonance, the unique, unstructured N-terminal domain of σA was identified to play a role in the σA-MoaB2 interaction. Functional experiments then showed that MoaB2 inhibits σA-dependent (but not σB-dependent) transcription and may increase the stability of σA in the cell. We propose that MoaB2, by sequestering σA, has a potential to modulate gene expression. In summary, this study has uncovered a new binding partner of mycobacterial σA, paving the way for future investigation of this phenomenon.

Přiložené soubory

https://is.muni.cz/publication/2480217/2480217.pdf

Citovat

BREZOVSKA, Barbora; Subhash NARASIMHAN; Michaela SIKOVA; Hana SANDEROVA; Tomas KOVAL; Nabajyoti BORAH; Mahmoud SHOMAN; Debora POSPISILOVA; Viola Vankova HAUSNEROVA; Dávid TUŽINČIN; Martin ČERNÝ; Jan KOMÁREK; Martina JANOUSKOVA; Milada KAMBOVA; Petr HALADA; Alena KRENKOVA; Martin HUBALEK; Maria TRUNDOVA; Jan DOHNALEK; Jarmila HNILICOVA; Lukáš ŽÍDEK a Libor KRASNY. MoaB2, a newly identified transcription factor, binds to σA in Mycobacterium smegmatis. Journal of Bacteriology. WASHINGTON: AMER SOC MICROBIOLOGY, 2024, roč. 206, č. 12, s. 1-30. ISSN 0021-9193. Dostupné z: https://dx.doi.org/10.1128/jb.00066-24.

@article{68767,
   author = {Brezovska, Barbora and Narasimhan, Subhash and Sikova, Michaela and Sanderova, Hana and Koval, Tomas and Borah, Nabajyoti and Shoman, Mahmoud and Pospisilova, Debora and Hausnerova, Viola Vankova and Tužinčin, Dávid and Černý, Martin and Komárek, Jan and Janouskova, Martina and Kambova, Milada and Halada, Petr and Krenkova, Alena and Hubalek, Martin and Trundova, Maria and Dohnalek, Jan and Hnilicova, Jarmila and Žídek, Lukáš and Krasny, Libor},
   article_location = {WASHINGTON},
   article_number = {12},
   doi = {http://dx.doi.org/10.1128/jb.00066-24},
   keywords = {MoaB2; sigma(A); mycobacteria; RNA polymerase; transcription},
   language = {eng},
   issn = {0021-9193},
   journal = {Journal of Bacteriology},
   title = {MoaB2, a newly identified transcription factor, binds to σA in Mycobacterium smegmatis},
   url = {https://doi.org/10.1128/jb.00066-24},
   volume = {206},
   year = {2024}
}

TY  - JOUR
ID  - 68767
AU  - Brezovska, Barbora - Narasimhan, Subhash - Sikova, Michaela - Sanderova, Hana - Koval, Tomas - Borah, Nabajyoti - Shoman, Mahmoud - Pospisilova, Debora - Hausnerova, Viola Vankova - Tužinčin, Dávid - Černý, Martin - Komárek, Jan - Janouskova, Martina - Kambova, Milada - Halada, Petr - Krenkova, Alena - Hubalek, Martin - Trundova, Maria - Dohnalek, Jan - Hnilicova, Jarmila - Žídek, Lukáš - Krasny, Libor
PY  - 2024
TI  - MoaB2, a newly identified transcription factor, binds to σA in Mycobacterium smegmatis
JF  - Journal of Bacteriology
VL  - 206
IS  - 12
SP  - 1-30
EP  - 1-30
PB  - AMER SOC MICROBIOLOGY
SN  - 0021-9193
KW  - MoaB2
KW  - sigma(A)
KW  - mycobacteria
KW  - RNA polymerase
KW  - transcription
UR  - https://doi.org/10.1128/jb.00066-24
N2  - In mycobacteria, σA is the primary sigma factor. This essential protein binds to RNA polymerase (RNAP) and mediates transcription initiation of housekeeping genes. Our knowledge about this factor in mycobacteria is limited. Here, we performed an unbiased search for interacting partners of Mycobacterium smegmatis σA. The search revealed a number of proteins; prominent among them was MoaB2. The σA-MoaB2 interaction was validated and characterized by several approaches, revealing that it likely does not require RNAP and is specific, as alternative σ factors (e.g., closely related σB) do not interact with MoaB2. The structure of MoaB2 was solved by X-ray crystallography. By immunoprecipitation and nuclear magnetic resonance, the unique, unstructured N-terminal domain of σA was identified to play a role in the σA-MoaB2 interaction. Functional experiments then showed that MoaB2 inhibits σA-dependent (but not σB-dependent) transcription and may increase the stability of σA in the cell. We propose that MoaB2, by sequestering σA, has a potential to modulate gene expression. In summary, this study has uncovered a new binding partner of mycobacterial σA, paving the way for future investigation of this phenomenon.
ER  -

BREZOVSKA, Barbora; Subhash NARASIMHAN; Michaela SIKOVA; Hana SANDEROVA; Tomas KOVAL; Nabajyoti BORAH; Mahmoud SHOMAN; Debora POSPISILOVA; Viola Vankova HAUSNEROVA; Dávid TUŽINČIN; Martin ČERNÝ; Jan KOMÁREK; Martina JANOUSKOVA; Milada KAMBOVA; Petr HALADA; Alena KRENKOVA; Martin HUBALEK; Maria TRUNDOVA; Jan DOHNALEK; Jarmila HNILICOVA; Lukáš ŽÍDEK a Libor KRASNY. MoaB2, a newly identified transcription factor, binds to σA in Mycobacterium smegmatis. \textit{Journal of Bacteriology}. WASHINGTON: AMER SOC MICROBIOLOGY, 2024, roč.~206, č.~12, s.~1-30. ISSN~0021-9193. Dostupné z: https://dx.doi.org/10.1128/jb.00066-24.