Přehled o publikaci
2024
Genome editing in almond using hairy root transformation system
JEDLIČKOVÁ, Veronika; Marie ŠTEFKOVÁ; Juan Francisco SANCHEZ LOPEZ; Jérôme GRIMPLET; María José RUBIO CABETAS et al.Basic information
Original name
Genome editing in almond using hairy root transformation system
Authors
JEDLIČKOVÁ, Veronika; Marie ŠTEFKOVÁ; Juan Francisco SANCHEZ LOPEZ; Jérôme GRIMPLET; María José RUBIO CABETAS and Helene ROBERT BOISIVON
Edition
Plant Cell, Tissue and Organ Culture (PCTOC), Springer, 2024, 0167-6857
Other information
Language
English
Type of outcome
Article in a journal
Country of publisher
Germany
Confidentiality degree
is not subject to a state or trade secret
References:
Marked to be transferred to RIV
Yes
RIV identification code
RIV/00216224:14740/24:00137955
Organization
Středoevropský technologický institut – Repository – Repository
UT WoS
EID Scopus
Keywords in English
Prunus dulcis; Hairy root culture; Composite plants; CRISPR/Cas9; ERF74; GAI
Links
EH22_008/0004581, research and development project. LTC20004, research and development project. Czech-BioImaging III, large research infrastructures.
Changed: 10/6/2025 00:50, RNDr. Daniel Jakubík
Abstract
In the original language
Woody plant species are often challenging to transform, which complicates gene function studies using molecular biology techniques. To develop an effective gene editing tool for almond (Prunus dulcis, syn. Prunus amygdalus), we established a new method for inducing hairy roots in almond seedlings based on the injection of agrobacterial culture. We generated composite plants comprising wild-type shoots and transgenic hairy roots. This approach was also successful in almond x peach hybrids. The hairy root transformation system was used alongside Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-associated protein 9 gene editing to target two transcription factor genes (ETHYLENE RESPONSE FACTOR 74 and GIBBERELLIC ACID INSENSITIVE). We successfully generated hairy roots with knockouts of the target genes and evaluated the efficiency of guide RNAs for gene editing in almond. Our transgenic root method could be a valuable tool for routine gene function studies in almond.
