The chapter analyses the performance of populist political parties in the 2024 EP election in the Czech Republic. The election ended with a significant increase in support for several populist parties: Action of Dissatisfied Citizens, Freedom and Direct Democracy and the Oath and Motorists. All populist parties used radical-right rhetoric before the election, expressing different levels of criticism of the European Union, strong anti-immigration attitudes and negative attitudes toward the Green Deal. The preliminary data show that the electoral support for the populists was based on a higher level of mobilization in so-called peripheral areas of the Czech Republic, potentially affected by recent inflation and austerity policies pursued by the government. All in all, the 2024 EP election in Czechia significantly increased support for populist political parties.