In-cell NMR suggests that DNA i-motif levels are strongly
depleted in living human cells

ENPřihlásit se Přihlásit se (EduID)

In-cell NMR suggests that DNA i-motif levels are strongly depleted in living human cells

Přehled o publikaci

J 2024

In-cell NMR suggests that DNA i-motif levels are strongly depleted in living human cells

VÍŠKOVÁ, Pavlína; Eva IŠTVÁNKOVÁ; Jan RYNEŠ; Šimon DŽATKO; Tomáš LOJA et. al.

Základní údaje

Originální název

In-cell NMR suggests that DNA i-motif levels are strongly depleted in living human cells

Autoři

Vydání

Nature Communications, London, Nature Publishing Group, 2024, 2041-1723

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Organizace

Středoevropský technologický institut – Masarykova univerzita – Repozitář

DOI

http://dx.doi.org/10.1038/s41467-024-46221-y

UT WoS

001180394600033

EID Scopus

2-s2.0-85186873110

Klíčová slova anglicky

CYTOSINE-RICH STRAND; G-QUADRUPLEX; HNRNP LL; MOLECULAR SWITCH; GENE-EXPRESSION; LOOP LENGTH; STABILITY; SEQUENCES; DOMAINS; PROTEIN

Návaznosti

EF18_046/0015974, projekt VaV. EF20_079/0017045, projekt VaV. GX19-26041X, projekt VaV. LM2023050, projekt VaV. LM2023053, projekt VaV. LX22NPO5102, projekt VaV. 871037, interní kód Repo. CIISB III, velká výzkumná infrastruktura. Czech-BioImaging III, velká výzkumná infrastruktura. EATRIS-CZ IV, velká výzkumná infrastruktura.

Změněno: 15. 3. 2025 00:51, RNDr. Daniel Jakubík

Anotace

V originále

gt; 7 appear as a mix of folded and unfolded states depending on the cell cycle phase. Comparing these results with previous data obtained using an iM-specific antibody (iMab) reveals that cell cycle-dependent iM formation has a dual origin, and iM formation concerns only a tiny fraction (possibly 1%) of genomic sites with iM formation propensity. We propose a comprehensive model aligning observations from iMab and in-cell NMR and enabling the identification of iMFPS capable of adopting iM structures under physiological conditions in living human cells. Our results suggest that many iMFPS may have biological roles linked to their unfolded states.

Přiložené soubory

https://is.muni.cz/publication/2409818/In-cell_NMR_suggests_that_DNA_i-motif_levels_are_strongly_depleted_in_living_human_cells.pdf

Citovat

VÍŠKOVÁ, Pavlína; Eva IŠTVÁNKOVÁ; Jan RYNEŠ; Šimon DŽATKO; Tomáš LOJA; Martina LENARČIČ ŽIVKOVIC; Riccardo RIGO; Roberto EL-KHOURY; Israel SERRANO-CHACON; Masad J DAMHA; Carlos GONZALEZ; Jean-Louis MERGNY; Silvie TRANTÍRKOVÁ a Lukáš TRANTÍREK. In-cell NMR suggests that DNA i-motif levels are strongly depleted in living human cells. Nature Communications. London: Nature Publishing Group, 2024, roč. 15, č. 1, s. 1-12. ISSN 2041-1723. Dostupné z: https://dx.doi.org/10.1038/s41467-024-46221-y.

@article{61574,
   author = {Víšková, Pavlína and Ištvánková, Eva and Ryneš, Jan and Džatko, Šimon and Loja, Tomáš and Lenarčič Živkovic, Martina and Rigo, Riccardo and ElandKhoury, Roberto and SerranoandChacon, Israel and Damha, Masad J and Gonzalez, Carlos and Mergny, JeanandLouis and Trantírková, Silvie and Trantírek, Lukáš},
   article_location = {London},
   article_number = {1},
   doi = {http://dx.doi.org/10.1038/s41467-024-46221-y},
   keywords = {CYTOSINE-RICH STRAND; G-QUADRUPLEX; HNRNP LL; MOLECULAR SWITCH; GENE-EXPRESSION; LOOP LENGTH; STABILITY; SEQUENCES; DOMAINS; PROTEIN},
   language = {eng},
   issn = {2041-1723},
   journal = {Nature Communications},
   title = {In-cell NMR suggests that DNA i-motif levels are strongly depleted in living human cells},
   url = {https://www.nature.com/articles/s41467-024-46221-y},
   volume = {15},
   year = {2024}
}

TY  - JOUR
ID  - 61574
AU  - Víšková, Pavlína - Ištvánková, Eva - Ryneš, Jan - Džatko, Šimon - Loja, Tomáš - Lenarčič Živkovic, Martina - Rigo, Riccardo - El-Khoury, Roberto - Serrano-Chacon, Israel - Damha, Masad J - Gonzalez, Carlos - Mergny, Jean-Louis - Trantírková, Silvie - Trantírek, Lukáš
PY  - 2024
TI  - In-cell NMR suggests that DNA i-motif levels are strongly depleted in living human cells
JF  - Nature Communications
VL  - 15
IS  - 1
SP  - 1-12
EP  - 1-12
PB  - Nature Publishing Group
SN  - 2041-1723
KW  - CYTOSINE-RICH STRAND
KW  - G-QUADRUPLEX
KW  - HNRNP LL
KW  - MOLECULAR SWITCH
KW  - GENE-EXPRESSION
KW  - LOOP LENGTH
KW  - STABILITY
KW  - SEQUENCES
KW  - DOMAINS
KW  - PROTEIN
UR  - https://www.nature.com/articles/s41467-024-46221-y
N2  - gt; 7 appear as a mix of folded and unfolded states depending on the cell cycle phase. Comparing these results with previous data obtained using an iM-specific antibody (iMab) reveals that cell cycle-dependent iM formation has a dual origin, and iM formation concerns only a tiny fraction (possibly 1%) of genomic sites with iM formation propensity. We propose a comprehensive model aligning observations from iMab and in-cell NMR and enabling the identification of iMFPS capable of adopting iM structures under physiological conditions in living human cells. Our results suggest that many iMFPS may have biological roles linked to their unfolded states.
ER  -

VÍŠKOVÁ, Pavlína; Eva IŠTVÁNKOVÁ; Jan RYNEŠ; Šimon DŽATKO; Tomáš LOJA; Martina LENARČIČ ŽIVKOVIC; Riccardo RIGO; Roberto EL-KHOURY; Israel SERRANO-CHACON; Masad J DAMHA; Carlos GONZALEZ; Jean-Louis MERGNY; Silvie TRANTÍRKOVÁ a Lukáš TRANTÍREK. In-cell NMR suggests that DNA i-motif levels are strongly depleted in living human cells. \textit{Nature Communications}. London: Nature Publishing Group, 2024, roč.~15, č.~1, s.~1-12. ISSN~2041-1723. Dostupné z: https://dx.doi.org/10.1038/s41467-024-46221-y.