Přehled o publikaci
2024
Global interactome mapping reveals pro-tumorigenic interactions of NF-κB in breast cancer
LAPČÍK, Petr; R. Greg STACEY; David POTĚŠIL; Petr KULHÁNEK; Leonard J. FOSTER et. al.Základní údaje
Originální název
Global interactome mapping reveals pro-tumorigenic interactions of NF-κB in breast cancer
Autoři
LAPČÍK, Petr; R. Greg STACEY; David POTĚŠIL; Petr KULHÁNEK; Leonard J. FOSTER a Pavel BOUCHAL
Vydání
Molecular and Cellular Proteomics, Elsevier, 2024, 1535-9484
Další údaje
Jazyk
angličtina
Typ výsledku
Článek v odborném periodiku
Stát vydavatele
Nizozemské království
Utajení
není předmětem státního či obchodního tajemství
Odkazy
Organizace
Přírodovědecká fakulta – Masarykova univerzita – Repozitář
UT WoS
001347387500001
EID Scopus
2-s2.0-85191897708
Klíčová slova anglicky
NF-κB; RELA; Proteomics; Protein complexes; Interaction; Breast cancer; Protein correlation profiling; AlphaPullDown
Návaznosti
LX22NPO5102, projekt VaV. NU22-08-00230, projekt VaV. CIISB III, velká výzkumná infrastruktura. e-INFRA CZ II, velká výzkumná infrastruktura.
Změněno: 25. 3. 2025 00:50, RNDr. Daniel Jakubík
Anotace
V originále
NF-κB pathway is involved in inflammation, however, recent data shows its role also in cancer development and progression, including metastasis. To understand the role of NF-κB interactome dynamics in cancer, we study the complexity of breast cancer interactome in luminal A breast cancer model and its rearrangement associated with NF-κB modulation. Liquid chromatography-mass spectrometry measurement of 160 size exclusion chromatography (SEC) fractions identifies 5460 protein groups. 7568 interactions among these proteins have been reconstructed by PrInCE algorithm, of which 2564 have been validated in independent datasets. NF-κB modulation leads to rearrangement of protein complexes involved in NF-κB signaling and immune response, cell cycle regulation and DNA replication. Central NF-κB transcription regulator RELA co-elutes with interactors of NF-κB activator PRMT5, and these complexes are confirmed by AlphaPulldown prediction. A complementary immunoprecipitation experiment recapitulates RELA interactions with other NF-κB factors, associating NF-κB inhibition with lower binding of NF-κB activators to RELA. This study describes a network of pro-tumorigenic protein interactions and their rearrangement upon NF-κB inhibition with potential therapeutic implications in tumors with high NF-κB activity.