Přehled o publikaci
2023
Enhanced in vitro culture of leukemic cells: Insights from collagen scaffolds and carboxymethyl cellulose-polyethylene glycol gel.
SVOZILOVÁ, Hana; Lucy VOJTOVÁ; Jana DORAZILOVÁ; Jana BRUKNEROVÁ; Veronika POLÁKOVÁ et al.Basic information
Original name
Enhanced in vitro culture of leukemic cells: Insights from collagen scaffolds and carboxymethyl cellulose-polyethylene glycol gel.
Authors
SVOZILOVÁ, Hana; Lucy VOJTOVÁ; Jana DORAZILOVÁ; Jana BRUKNEROVÁ; Veronika POLÁKOVÁ; Lenka RADOVÁ; Michael DOUBEK; Karla PLEVOVÁ and Šárka POSPÍŠILOVÁ
Edition
EMBL Conference Cancer Genomics 2023, Heidelberg, Německo, 2023
Other information
Language
English
Type of outcome
Konferenční abstrakta
Country of publisher
Germany
Confidentiality degree
is not subject to a state or trade secret
References:
Marked to be transferred to RIV
Yes
RIV identification code
RIV/00216224:14740/23:00132768
Organization
Středoevropský technologický institut – Repository – Repository
Keywords in English
chronic lymphocytic leukemia; cell-to-cell; cell-to-matrix contacts
Links
LM2018129, research and development project. LM2023067, research and development project. MUNI/A/1224/2022, interní kód Repo.
Changed: 23/12/2025 00:51, RNDr. Daniel Jakubík
Abstract
In the original language
Studying chronic lymphocytic leukemia (CLL) in vitro is challenging due to its complexity and dependency of CLL cells on their microenvironment. An integral part of the natural CLL microenvironment is the three-dimensional (3D) spatial organization, which facilitates frequent cell-to-cell and cell-to-matrix contacts. Therefore, we aimed to mimic the natural tissue architecture by implementing a 3D in vitro culture. We hypothesized that compared to conventional culture, the additional dimension and increased cell-to-matrix contacts would enhance the prosurvival stimuli in CLL cells. We cultured CLL cells in two materials: collagen scaffolds, or gel composed of carboxymethyl cellulose and polyethylene glycol (CMC-PEG). We assessed cell distribution, morphology, and viability via microscopy, and measured the metabolic activity by AlamarBlue assay. Gene expression (MYC, VCAM1, MCL1, CXCR4, CCL4) was analyzed using qPCR to understand the effects of novel culture approaches on adhesion, apoptosis, and intercellular interactions of CLL cells co-cultured with bone marrow stromal cells (BMSCs) in 3D.
