This study describes the process of translating, adapting and validating the Klein et al’s Unidimensional Target-neutral Scale of Commitment (KUT) into Czech. The KUT scale is a self-report scale that consists of four items. The scale was adapted using three independent translations into Czech, one back-translation into English by a native speaker, assessment by experts, and piloting in cognitive interviews with 13 respondents. The study also provides evidence on the scale’s reliability and validity for measuring organizational commitment defined as a psychological bond with an organization. The analyses were based on data obtained from 811 Czech-speaking adults working for Czech organizations. Using ordinal structural equation models (SEM) estimated on the polychoric correlation matrices with the robust WLSMV estimator, the Czech adaptation of the KUT scale showed similar psychometric characteristics to the original English version. The Czech KUT scale is unidimensional and internally consistent. All items have high factor loadings. The scale is invariant across groups defined by gender, workload and the sector in which the organization operates. The convergent validity is supported by the fact that the organizational commitment measured by the Czech KUT scale relates positively to job satisfaction, self-rating of job performance, extra-role behaviour and negatively to turnover intention. The Czech KUT scale also proved incremental validity to job performance over job satisfaction measured by Job Descriptive Survey. Therefore, the scale is a valid instrument for measuring organizational commitment within the Czech-speaking population. It can be used for organizational surveys as well as for research within the organizations.