Disclosing quantitative RT-PCR raw data during manuscript
submission: a call for action

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Disclosing quantitative RT-PCR raw data during manuscript submission: a call for action

Přehled o publikaci

J 2023

Disclosing quantitative RT-PCR raw data during manuscript submission: a call for action

UNTERGASSER, Andreas, Jan HELLEMANS, Michael W PFAFFL, Jan M RUIJTER, Maurice J B VAN DEN HOFF et. al.

Basic information

Original name

Disclosing quantitative RT-PCR raw data during manuscript submission: a call for action

Authors

Edition

MOLECULAR ONCOLOGY, ENGLAND, WILEY, 2023, 1574-7891

Other information

Language

English

Type of outcome

Article in a journal

Country of publisher

United States of America

Confidentiality degree

is not subject to a state or trade secret

References:

URL

Organization

Lékařská fakulta – Repository – Repository

DOI

http://dx.doi.org/10.1002/1878-0261.13418

UT WoS

000982873500001

EID Scopus

2-s2.0-85151090211

Keywords in English

accuracy; quantification; RNA; RT-qPCR

Links

LX22NPO5102, research and development project.

Changed: 29/2/2024 04:00, RNDr. Daniel Jakubík

Abstract

V originále

Accuracy and transparency of scientific data are becoming more and more relevant with the increasing concern regarding the evaluation of data reproducibility in many research areas. This concern is also true for quantifying coding and noncoding RNAs, with the remarkable increase in publications reporting RNA profiling and sequencing studies. To address the problem, we propose the following recommendations: (a) accurate documentation of experimental procedures in Materials and methods (and not only in the supplementary information, as many journals have a strict mandate for making Materials and methods as visible as possible in the main text); (b) submission of RT-qPCR raw data for all experiments reported; and (c) adoption of a unified, simple format for submitted RT-qPCR raw data. The Real-time PCR Data Essential Spreadsheet Format (RDES) was created for this purpose.

Files attached

https://is.muni.cz/publication/2348557/Disclosing_quantitative_RT-PCR_raw_data_during_manuscript_submission_a_call_for_action.pdf

Cite

UNTERGASSER, Andreas, Jan HELLEMANS, Michael W PFAFFL, Jan M RUIJTER, Maurice J B VAN DEN HOFF, Mihnea P DRAGOMIR, Douglas ADAMOSKI, Sandra Martha Gomes DIAS, Rui Manuel REIS, Manuela FERRACIN, Emmanuel DIAS-NETO, Ian MARSH, Mikael KUBISTA, Muller FABBRI, Ajay GOEL, Ondřej SLABÝ, Erik KNUTSEN, Baoqing CHEN, Massimo NEGRINI, Koshi MIMORI, Martin PICHLER, Maria PAPATRIANTAFYLLOU, Simone ANFOSSI, Thomas D SCHMITTGEN, Jim HUGGETT, Stephen BUSTIN, Jo VANDESOMPELE and George A CALIN. Disclosing quantitative RT-PCR raw data during manuscript submission: a call for action. MOLECULAR ONCOLOGY. ENGLAND: WILEY, 2023, vol. 17, No 5, p. 713-717. ISSN 1574-7891. Available from: https://dx.doi.org/10.1002/1878-0261.13418.

@article{58693,
   author = {Untergasser, Andreas and Hellemans, Jan and Pfaffl, Michael W and Ruijter, Jan M and van den Hoff, Maurice J B and Dragomir, Mihnea P and Adamoski, Douglas and Dias, Sandra Martha Gomes and Reis, Rui Manuel and Ferracin, Manuela and DiasandNeto, Emmanuel and Marsh, Ian and Kubista, Mikael and Fabbri, Muller and Goel, Ajay and Slabý, Ondřej and Knutsen, Erik and Chen, Baoqing and Negrini, Massimo and Mimori, Koshi and Pichler, Martin and Papatriantafyllou, Maria and Anfossi, Simone and Schmittgen, Thomas D and Huggett, Jim and Bustin, Stephen and Vandesompele, Jo and Calin, George A},
   article_location = {ENGLAND},
   article_number = {5},
   doi = {http://dx.doi.org/10.1002/1878-0261.13418},
   keywords = {accuracy; quantification; RNA; RT-qPCR},
   language = {eng},
   issn = {1574-7891},
   journal = {MOLECULAR ONCOLOGY},
   title = {Disclosing quantitative RT-PCR raw data during manuscript submission: a call for action},
   url = {https://febs.onlinelibrary.wiley.com/doi/10.1002/1878-0261.13418},
   volume = {17},
   year = {2023}
}

TY  - JOUR
ID  - 58693
AU  - Untergasser, Andreas - Hellemans, Jan - Pfaffl, Michael W - Ruijter, Jan M - van den Hoff, Maurice J B - Dragomir, Mihnea P - Adamoski, Douglas - Dias, Sandra Martha Gomes - Reis, Rui Manuel - Ferracin, Manuela - Dias-Neto, Emmanuel - Marsh, Ian - Kubista, Mikael - Fabbri, Muller - Goel, Ajay - Slabý, Ondřej - Knutsen, Erik - Chen, Baoqing - Negrini, Massimo - Mimori, Koshi - Pichler, Martin - Papatriantafyllou, Maria - Anfossi, Simone - Schmittgen, Thomas D - Huggett, Jim - Bustin, Stephen - Vandesompele, Jo - Calin, George A
PY  - 2023
TI  - Disclosing quantitative RT-PCR raw data during manuscript submission: a call for action
JF  - MOLECULAR ONCOLOGY
VL  - 17
IS  - 5
SP  - 713-717
EP  - 713-717
PB  - WILEY
SN  - 1574-7891
KW  - accuracy
KW  - quantification
KW  - RNA
KW  - RT-qPCR
UR  - https://febs.onlinelibrary.wiley.com/doi/10.1002/1878-0261.13418
N2  - Accuracy and transparency of scientific data are becoming more and more relevant with the increasing concern regarding the evaluation of data reproducibility in many research areas. This concern is also true for quantifying coding and noncoding RNAs, with the remarkable increase in publications reporting RNA profiling and sequencing studies. To address the problem, we propose the following recommendations: (a) accurate documentation of experimental procedures in Materials and methods (and not only in the supplementary information, as many journals have a strict mandate for making Materials and methods as visible as possible in the main text); (b) submission of RT-qPCR raw data for all experiments reported; and (c) adoption of a unified, simple format for submitted RT-qPCR raw data. The Real-time PCR Data Essential Spreadsheet Format (RDES) was created for this purpose.
ER  -

UNTERGASSER, Andreas, Jan HELLEMANS, Michael W PFAFFL, Jan M RUIJTER, Maurice J B VAN DEN HOFF, Mihnea P DRAGOMIR, Douglas ADAMOSKI, Sandra Martha Gomes DIAS, Rui Manuel REIS, Manuela FERRACIN, Emmanuel DIAS-NETO, Ian MARSH, Mikael KUBISTA, Muller FABBRI, Ajay GOEL, Ondřej SLABÝ, Erik KNUTSEN, Baoqing CHEN, Massimo NEGRINI, Koshi MIMORI, Martin PICHLER, Maria PAPATRIANTAFYLLOU, Simone ANFOSSI, Thomas D SCHMITTGEN, Jim HUGGETT, Stephen BUSTIN, Jo VANDESOMPELE and George A CALIN. Disclosing quantitative RT-PCR raw data during manuscript submission: a call for action. \textit{MOLECULAR ONCOLOGY}. ENGLAND: WILEY, 2023, vol.~17, No~5, p.~713-717. ISSN~1574-7891. Available from: https://dx.doi.org/10.1002/1878-0261.13418.