J
2023
A thermosensitive gel matrix for bioreactor-assisted in-cell NMR of nucleic acids and proteins
DZUROV, Matej; Šárka POSPÍŠILOVÁ; Michaela KRAFČÍKOVÁ; Lukáš TRANTÍREK; Lucy VOJTOVÁ et al.
Basic information
Original name
A thermosensitive gel matrix for bioreactor-assisted in-cell NMR of nucleic acids and proteins
Authors
DZUROV, Matej; Šárka POSPÍŠILOVÁ; Michaela KRAFČÍKOVÁ; Lukáš TRANTÍREK; Lucy VOJTOVÁ and Jan RYNEŠ
Edition
Journal of biomolecular NMR, Dordrecht, Springer, 2023, 0925-2738
Other information
Type of outcome
Article in a journal
Country of publisher
Netherlands
Confidentiality degree
is not subject to a state or trade secret
Marked to be transferred to RIV
Yes
RIV identification code
RIV/00216224:14740/23:00132257
Organization
Středoevropský technologický institut – Repository – Repository
Keywords in English
PLA-PEG-PLA; Thermosensitive hydrogel; Cell immobilization; Bioreactor; In-cell NMR
Links
LM2023053, research and development project. LX22NPO5102, research and development project. NV19-08-00450, research and development project. 871037, interní kód Repo. CzeCOS III, large research infrastructures. CIISB II, large research infrastructures. Czech-BioImaging II, large research infrastructures.
In the original language
amp; DEG;C and, importantly, allow recovery of cells after completion of in-cell NMR spectra acquisition. We compared the performances of currently used matrices in terms of cell viability (dye exclusion assays), cellular metabolism (1D 31P NMR), and quality of in-cell NMR spectra of two model biomacromolecules (hybrid double-stranded/i-motif DNA and ubiquitin). Our results demonstrate the suitability and advantages of PLA-PEG-PLA copolymers for application in bioreactor-assisted in-cell NMR.
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