Titanium Dioxide Nanoparticles Modulate Systemic Immune
Response and Increase Levels of Reduced Glutathione in Mice
after Seven-Week Inhalation

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Titanium Dioxide Nanoparticles Modulate Systemic Immune Response and Increase Levels of Reduced ...

Přehled o publikaci

J 2023

Titanium Dioxide Nanoparticles Modulate Systemic Immune Response and Increase Levels of Reduced Glutathione in Mice after Seven-Week Inhalation

MIROSLAVA, Lehotska Mikusova; Milena BUSOVA; Jana TULINSKA; Vlasta MASANOVA; Aurelia LISKOVA et. al.

Basic information

Original name

Titanium Dioxide Nanoparticles Modulate Systemic Immune Response and Increase Levels of Reduced Glutathione in Mice after Seven-Week Inhalation

Authors

Edition

Nanomaterials, MDPI, 2023, 2079-4991

Other information

Language

English

Type of outcome

Article in a journal

Country of publisher

Switzerland

Confidentiality degree

is not subject to a state or trade secret

References:

URL

Organization

Přírodovědecká fakulta – Repository – Repository

DOI

http://dx.doi.org/10.3390/nano13040767

UT WoS

000939931900001

EID Scopus

2-s2.0-85149031929

Keywords in English

titanium dioxide nanoparticles; nanoparticle inhalation; immunotoxicity; immune response; lymphocytes; cytokines; inflammation; phagocytic activity and respiratory burst; antioxidant defense

Links

EF15_003/0000469, research and development project. EF17_043/0009632, research and development project. 857560, interní kód Repo. RECETOX RI, large research infrastructures.

Changed: 10/3/2024 03:40, RNDr. Daniel Jakubík

Abstract

V originále

Titanium dioxide nanoparticles (TiO2 NPs) are used in a wide range of applications. Although inhalation of NPs is one of the most important toxicologically relevant routes, experimental studies on potential harmful effects of TiO2 NPs using a whole-body inhalation chamber model are rare. In this study, the profile of lymphocyte markers, functional immunoassays, and antioxidant defense markers were analyzed to evaluate the potential adverse effects of seven-week inhalation exposure to two different concentrations of TiO2 NPs (0.00167 and 0.1308 mg TiO2/m(3)) in mice. A dose-dependent effect of TiO2 NPs on innate immunity was evident in the form of stimulated phagocytic activity of monocytes in low-dose mice and suppressed secretory function of monocytes (IL-18) in high-dose animals. The effect of TiO2 NPs on adaptive immunity, manifested in the spleen by a decrease in the percentage of T-cells, a reduction in T-helper cells, and a dose-dependent decrease in lymphocyte cytokine production, may indicate immunosuppression in exposed mice. The dose-dependent increase in GSH concentration and GSH/GSSG ratio in whole blood demonstrated stimulated antioxidant defense against oxidative stress induced by TiO2 NP exposure.

Files attached

https://is.muni.cz/publication/2295460/2295460.pdf

Cite

MIROSLAVA, Lehotska Mikusova; Milena BUSOVA; Jana TULINSKA; Vlasta MASANOVA; Aurelia LISKOVA; Iveta UHNAKOVA; Maria DUSINSKA; Zora KRIVOSIKOVA; Eva ROLLEROVA; Radka ALACOVA; Ladislava WSOLOVA; Mira HORVATHOVA; Michaela SZABOVA; Norbert LUKAN; Zbynek VECERA; Pavel COUFALIK; Kamil KRUMAL; Lukas ALEXA; Vojtěch THON; Pavel PILER; Marcela BUCHTOVÁ; Lucie VRLÍKOVÁ; Pavel MORAVEC; Dusan GALANDA and Pavel MIKUSKA. Titanium Dioxide Nanoparticles Modulate Systemic Immune Response and Increase Levels of Reduced Glutathione in Mice after Seven-Week Inhalation. Nanomaterials. MDPI, 2023, vol. 13, No 4, p. 1-16. ISSN 2079-4991. Available from: https://dx.doi.org/10.3390/nano13040767.

@article{56647,
   author = {Miroslava, Lehotska Mikusova and Busova, Milena and Tulinska, Jana and Masanova, Vlasta and Liskova, Aurelia and Uhnakova, Iveta and Dusinska, Maria and Krivosikova, Zora and Rollerova, Eva and Alacova, Radka and Wsolova, Ladislava and Horvathova, Mira and Szabova, Michaela and Lukan, Norbert and Vecera, Zbynek and Coufalik, Pavel and Krumal, Kamil and Alexa, Lukas and Thon, Vojtěch and Piler, Pavel and Buchtová, Marcela and Vrlíková, Lucie and Moravec, Pavel and Galanda, Dusan and Mikuska, Pavel},
   article_number = {4},
   doi = {http://dx.doi.org/10.3390/nano13040767},
   keywords = {titanium dioxide nanoparticles; nanoparticle inhalation; immunotoxicity; immune response; lymphocytes; cytokines; inflammation; phagocytic activity and respiratory burst; antioxidant defense},
   language = {eng},
   issn = {2079-4991},
   journal = {Nanomaterials},
   title = {Titanium Dioxide Nanoparticles Modulate Systemic Immune Response and Increase Levels of Reduced Glutathione in Mice after Seven-Week Inhalation},
   url = {https://www.mdpi.com/2079-4991/13/4/767},
   volume = {13},
   year = {2023}
}

TY  - JOUR
ID  - 56647
AU  - Miroslava, Lehotska Mikusova - Busova, Milena - Tulinska, Jana - Masanova, Vlasta - Liskova, Aurelia - Uhnakova, Iveta - Dusinska, Maria - Krivosikova, Zora - Rollerova, Eva - Alacova, Radka - Wsolova, Ladislava - Horvathova, Mira - Szabova, Michaela - Lukan, Norbert - Vecera, Zbynek - Coufalik, Pavel - Krumal, Kamil - Alexa, Lukas - Thon, Vojtěch - Piler, Pavel - Buchtová, Marcela - Vrlíková, Lucie - Moravec, Pavel - Galanda, Dusan - Mikuska, Pavel
PY  - 2023
TI  - Titanium Dioxide Nanoparticles Modulate Systemic Immune Response and Increase Levels of Reduced Glutathione in Mice after Seven-Week Inhalation
JF  - Nanomaterials
VL  - 13
IS  - 4
SP  - 1-16
EP  - 1-16
PB  - MDPI
SN  - 2079-4991
KW  - titanium dioxide nanoparticles
KW  - nanoparticle inhalation
KW  - immunotoxicity
KW  - immune response
KW  - lymphocytes
KW  - cytokines
KW  - inflammation
KW  - phagocytic activity and respiratory burst
KW  - antioxidant defense
UR  - https://www.mdpi.com/2079-4991/13/4/767
N2  - Titanium dioxide nanoparticles (TiO2 NPs) are used in a wide range of applications. Although inhalation of NPs is one of the most important toxicologically relevant routes, experimental studies on potential harmful effects of TiO2 NPs using a whole-body inhalation chamber model are rare. In this study, the profile of lymphocyte markers, functional immunoassays, and antioxidant defense markers were analyzed to evaluate the potential adverse effects of seven-week inhalation exposure to two different concentrations of TiO2 NPs (0.00167 and 0.1308 mg TiO2/m(3)) in mice. A dose-dependent effect of TiO2 NPs on innate immunity was evident in the form of stimulated phagocytic activity of monocytes in low-dose mice and suppressed secretory function of monocytes (IL-18) in high-dose animals. The effect of TiO2 NPs on adaptive immunity, manifested in the spleen by a decrease in the percentage of T-cells, a reduction in T-helper cells, and a dose-dependent decrease in lymphocyte cytokine production, may indicate immunosuppression in exposed mice. The dose-dependent increase in GSH concentration and GSH/GSSG ratio in whole blood demonstrated stimulated antioxidant defense against oxidative stress induced by TiO2 NP exposure.
ER  -

MIROSLAVA, Lehotska Mikusova; Milena BUSOVA; Jana TULINSKA; Vlasta MASANOVA; Aurelia LISKOVA; Iveta UHNAKOVA; Maria DUSINSKA; Zora KRIVOSIKOVA; Eva ROLLEROVA; Radka ALACOVA; Ladislava WSOLOVA; Mira HORVATHOVA; Michaela SZABOVA; Norbert LUKAN; Zbynek VECERA; Pavel COUFALIK; Kamil KRUMAL; Lukas ALEXA; Vojtěch THON; Pavel PILER; Marcela BUCHTOVÁ; Lucie VRLÍKOVÁ; Pavel MORAVEC; Dusan GALANDA and Pavel MIKUSKA. Titanium Dioxide Nanoparticles Modulate Systemic Immune Response and Increase Levels of Reduced Glutathione in Mice after Seven-Week Inhalation. \textit{Nanomaterials}. MDPI, 2023, vol.~13, No~4, p.~1-16. ISSN~2079-4991. Available from: https://dx.doi.org/10.3390/nano13040767.