Electron microscopy reveals toroidal shape of master neuronal
cell differentiator REST – RE1-silencing transcription factor

Přehled o publikaci

J 2023

Electron microscopy reveals toroidal shape of master neuronal cell differentiator REST – RE1-silencing transcription factor

VEVERKA, Pavel; Tomáš BROM; Tomáš JANOVIČ; Martin STOJASPAL; Matyáš PINKAS et al.

Basic information

Original name

Electron microscopy reveals toroidal shape of master neuronal cell differentiator REST – RE1-silencing transcription factor

Authors

VEVERKA, Pavel; Tomáš BROM; Tomáš JANOVIČ; Martin STOJASPAL; Matyáš PINKAS; Jiří NOVÁČEK and Ctirad HOFR

Edition

Computational and Structural Biotechnology Journal, Amsterdam, Elsevier, 2023, 2001-0370

Other information

Language

English

Type of outcome

Article in a journal

Country of publisher

Czech Republic

Confidentiality degree

is not subject to a state or trade secret

References:

URL

Marked to be transferred to RIV

Yes

RIV identification code

RIV/00216224:14310/23:00130199

Organization

Přírodovědecká fakulta – Repository – Repository

Keywords in English

REST; RE1-silencing transcription factor; Neuron-restrictive silencer factor; Neuronal Differentiation; REST-N62; REST4; NRSF; EM; Electron microscopy

Links

GA19-18226S, research and development project. LM2018127, research and development project. LTAUSA19024, research and development project.

Changed: 9/12/2025 00:50, RNDr. Daniel Jakubík

Abstract

In the original language

The RE1-Silencing Transcription factor (REST) is essential for neuronal differentiation. Here, we report the first 18.5-angstrom electron microscopy structure of human REST. The refined electron map suggests that REST forms a torus that can accommodate DNA double-helix in the central hole. Additionally, we quantitatively described REST binding to the canonical DNA sequence of the neuron-restrictive silencer element. We developed protocols for the expression and purification of full-length REST and the shortened variant REST-N62 produced by alternative splicing. We tested the mutual interaction of full-length REST and the splicing variant REST-N62. Revealed structure-function relationships of master neuronal repressor REST will allow finding new biological ways of prevention and treatment of neurodegenerative disorders and diseases.

Files attached

https://is.muni.cz/publication/2247909/2022_REST_Veverka.pdf

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Cite

VEVERKA, Pavel; Tomáš BROM; Tomáš JANOVIČ; Martin STOJASPAL; Matyáš PINKAS; Jiří NOVÁČEK and Ctirad HOFR. Electron microscopy reveals toroidal shape of master neuronal cell differentiator REST – RE1-silencing transcription factor. Computational and Structural Biotechnology Journal. Amsterdam: Elsevier, 2023, vol. 21, Dec, p. 731-741. ISSN 2001-0370. Available from: https://doi.org/10.1016/j.csbj.2022.12.026.

@article{54872,
   author = {Veverka, Pavel and Brom, Tomáš and Janovič, Tomáš and Stojaspal, Martin and Pinkas, Matyáš and Nováček, Jiří and Hofr, Ctirad},
   article_location = {Amsterdam},
   article_number = {Dec},
   doi = {https://doi.org/10.1016/j.csbj.2022.12.026},
   keywords = {REST; RE1-silencing transcription factor; Neuron-restrictive silencer factor; Neuronal Differentiation; REST-N62; REST4; NRSF; EM; Electron microscopy},
   language = {eng},
   issn = {2001-0370},
   journal = {Computational and Structural Biotechnology Journal},
   title = {Electron microscopy reveals toroidal shape of master neuronal cell differentiator REST – RE1-silencing transcription factor},
   url = {https://www.sciencedirect.com/science/article/pii/S2001037022005827},
   volume = {21},
   year = {2023}
}

TY  - JOUR
ID  - 54872
AU  - Veverka, Pavel - Brom, Tomáš - Janovič, Tomáš - Stojaspal, Martin - Pinkas, Matyáš - Nováček, Jiří - Hofr, Ctirad
PY  - 2023
TI  - Electron microscopy reveals toroidal shape of master neuronal cell differentiator REST – RE1-silencing transcription factor
JF  - Computational and Structural Biotechnology Journal
VL  - 21
IS  - Dec
SP  - 731-741
EP  - 731-741
PB  - Elsevier
SN  - 2001-0370
KW  - REST
KW  - RE1-silencing transcription factor
KW  - Neuron-restrictive silencer factor
KW  - Neuronal Differentiation
KW  - REST-N62
KW  - REST4
KW  - NRSF
KW  - EM
KW  - Electron microscopy
UR  - https://www.sciencedirect.com/science/article/pii/S2001037022005827
N2  - The RE1-Silencing Transcription factor (REST) is essential for neuronal differentiation. Here, we report the first 18.5-angstrom electron microscopy structure of human REST. The refined electron map suggests that REST forms a torus that can accommodate DNA double-helix in the central hole. Additionally, we quantitatively described REST binding to the canonical DNA sequence of the neuron-restrictive silencer element. We developed protocols for the expression and purification of full-length REST and the shortened variant REST-N62 produced by alternative splicing. We tested the mutual interaction of full-length REST and the splicing variant REST-N62. Revealed structure-function relationships of master neuronal repressor REST will allow finding new biological ways of prevention and treatment of neurodegenerative disorders and diseases.
ER  -

VEVERKA, Pavel; Tomáš BROM; Tomáš JANOVIČ; Martin STOJASPAL; Matyáš PINKAS; Jiří NOVÁČEK and Ctirad HOFR. Electron microscopy reveals toroidal shape of master neuronal cell differentiator REST – RE1-silencing transcription factor. \textit{Computational and Structural Biotechnology Journal}. Amsterdam: Elsevier, 2023, vol.~21, Dec, p.~731-741. ISSN~2001-0370. Available from: https://doi.org/10.1016/j.csbj.2022.12.026.

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