2019
Engineering enzyme access tunnels
KOKKONEN, Piia Pauliina; David BEDNÁŘ; G. PINTO; Zbyněk PROKOP; Jiří DAMBORSKÝ et. al.Basic information
Original name
Engineering enzyme access tunnels
Authors
KOKKONEN, Piia Pauliina (246 Finland, belonging to the institution); David BEDNÁŘ (203 Czech Republic, belonging to the institution); G. PINTO; Zbyněk PROKOP (203 Czech Republic, belonging to the institution) and Jiří DAMBORSKÝ (203 Czech Republic, guarantor, belonging to the institution)
Edition
BIOTECHNOLOGY ADVANCES, OXFORD, PERGAMON-ELSEVIER SCIENCE LTD, 2019, 0734-9750
Other information
Language
English
Type of outcome
Article in a journal
Country of publisher
United Kingdom of Great Britain and Northern Ireland
Confidentiality degree
is not subject to a state or trade secret
References:
RIV identification code
RIV/00216224:14310/19:00113351
Organization
Přírodovědecká fakulta – Repository – Repository
UT WoS
000484647000003
EID Scopus
2-s2.0-85065027212
Keywords in English
Tunnel; Channel; Ligand binding; Pathway; Pore; Product release; Protein design; Protein engineering; Substrate entry; Dynamics
Links
EF16_013/0001761, research and development project. EF17_050/0008496, research and development project. LM2015047, research and development project. LM2015055, research and development project. LM2015085, research and development project.
Changed: 16/2/2023 04:23, RNDr. Daniel Jakubík
Abstract
V originále
Enzymes are efficient and specific catalysts for many essential reactions in biotechnological and pharmaceutical industries. Many times, the natural enzymes do not display the catalytic efficiency, stability or specificity required for these industrial processes. The current enzyme engineering methods offer solutions to this problem, but they mainly target the buried active site where the chemical reaction takes place. Despite being many times ignored, the tunnels and channels connecting the environment with the active site are equally important for the catalytic properties of enzymes. Changes in the enzymatic tunnels and channels affect enzyme activity, specificity, promiscuity, enantioselectivity and stability. This review provides an overview of the emerging field of enzyme access tunnel engineering with case studies describing design of all the aforementioned properties. The software tools for the analysis of geometry and function of the enzymatic tunnels and channels and for the rational design of tunnel modifications will also be discussed. The combination of new software tools and enzyme engineering strategies will provide enzymes with access tunnels and channels specifically tailored for individual industrial processes.
