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@article{53771,
author = {Pospíšil, Jakub and Hrabovsky, Milos and Bohačiaková, Dáša and Hovadkova, Zuzana and Jurásek, Miroslav and Mlčoušková, Jarmila and Paruch, Kamil and Nevolová, Šárka and Damborský, Jiří and Hampl, Aleš and Jaroš, Josef},
article_location = {WASHINGTON},
article_number = {11},
doi = {http://dx.doi.org/10.1021/acsbiomaterials.2c00650},
keywords = {nanopatterning; nanospacing; biomimetic surface; electron-beam lithography; cell-cell interaction; cell adhesion and spreading; ligand clustering},
language = {eng},
issn = {2373-9878},
journal = {ENGINEERING},
title = {Geometric Control of Cell Behavior by Biomolecule Nanodistribution},
url = {utm_medium=twitter},
volume = {8},
year = {2022}
}
TY - JOUR
ID - 53771
AU - Pospíšil, Jakub - Hrabovsky, Milos - Bohačiaková, Dáša - Hovadkova, Zuzana - Jurásek, Miroslav - Mlčoušková, Jarmila - Paruch, Kamil - Nevolová, Šárka - Damborský, Jiří - Hampl, Aleš - Jaroš, Josef
PY - 2022
TI - Geometric Control of Cell Behavior by Biomolecule Nanodistribution
JF - ENGINEERING
VL - 8
IS - 11
SP - 4789-4806
EP - 4789-4806
PB - AMER CHEMICAL SOC
SN - 2373-9878
KW - nanopatterning
KW - nanospacing
KW - biomimetic surface
KW - electron-beam lithography
KW - cell-cell interaction
KW - cell adhesion and spreading
KW - ligand clustering
UR - utm_medium=twitter
N2 - Many dynamic interactions within the cell micro-environment modulate cell behavior and cell fate. However, the pathways and mechanisms behind cell-cell or cell-extracellular matrix interactions remain understudied, as they occur at a nanoscale level. Recent progress in nanotechnology allows for mimicking of the microenvironment at nanoscale in vitro; electron-beam lithography (EBL) is currently the most promising technique. Although this nanopatterning technique can generate nanostructures of good quality and resolution, it has resulted, thus far, in the production of only simple shapes (e.g., rectangles) over a relatively small area (100 x 100 mu m), leaving its potential in biological applications unfulfilled. Here, we used EBL for cell-interaction studies by coating cell-culture-relevant material with electron-conductive indium tin oxide, which formed nanopatterns of complex nanohexagonal structures over a large area (500 x 500 mu m). We confirmed the potential of EBL for use in cell-interaction studies by analyzing specific cell responses toward differentially distributed nanohexagons spaced at 1000, 500, and 250 nm. We found that our optimized technique of EBL with HaloTags enabled the investigation of broad changes to a cell-culture-relevant surface and can provide an understanding of cellular signaling mechanisms at a single-molecule level.
ER -
POSPÍŠIL, Jakub, Milos HRABOVSKY, Dáša BOHAČIAKOVÁ, Zuzana HOVADKOVA, Miroslav JURÁSEK, Jarmila MLČOUŠKOVÁ, Kamil PARUCH, Šárka NEVOLOVÁ, Jiří DAMBORSKÝ, Aleš HAMPL a Josef JAROŠ. Geometric Control of Cell Behavior by Biomolecule Nanodistribution. \textit{ENGINEERING}. WASHINGTON: AMER CHEMICAL SOC, 2022, roč.~8, č.~11, s.~4789-4806. ISSN~2373-9878. Dostupné z: https://dx.doi.org/10.1021/acsbiomaterials.2c00650.
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