Copper Oxide Nanoparticles Stimulate the Immune Response and
Decrease Antioxidant Defense in Mice After Six-Week Inhalation

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Copper Oxide Nanoparticles Stimulate the Immune Response and Decrease Antioxidant Defense in Mice ...

Přehled o publikaci

J 2022

Copper Oxide Nanoparticles Stimulate the Immune Response and Decrease Antioxidant Defense in Mice After Six-Week Inhalation

TULINSKA, Jana, Miroslava Lehotska MIKUSOVA, Aurelia LISKOVA, Milena BUSOVA, Vlasta MASANOVA et. al.

Základní údaje

Originální název

Copper Oxide Nanoparticles Stimulate the Immune Response and Decrease Antioxidant Defense in Mice After Six-Week Inhalation

Autoři

TULINSKA, Jana, Miroslava Lehotska MIKUSOVA, Aurelia LISKOVA, Milena BUSOVA, Vlasta MASANOVA, Iveta UHNAKOVA, Eva ROLLEROVA, Radka ALACOVA, Zora KRIVOSIKOVA, Ladislava WSOLOVA, Maria DUSINSKA, Mira HORVATHOVA, Michaela SZABOVA, Norbert LUKAN, Martina STUCHLIKOVA, Daniel KUBA, Zbynek VECERA, Pavel COUFALIK, Kamil KRUMAL, Lukas ALEXA, Lucie VRLIKOVA, Marcela BUCHTOVÁ (203 Česká republika), Jana DUMKOVÁ (203 Česká republika, domácí), Pavel PILER (203 Česká republika, domácí), Vojtěch THON (203 Česká republika, garant, domácí) a Pavel MIKUSKA

Vydání

Frontiers in Immunology, LAUSANNE, FRONTIERS MEDIA SA, 2022, 1664-3224

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Kód RIV

RIV/00216224:14310/22:00127548

Organizace

Přírodovědecká fakulta – Masarykova univerzita – Repozitář

DOI

http://dx.doi.org/10.3389/fimmu.2022.874253

UT WoS

000805885900001

EID Scopus

2-s2.0-85129831954

Klíčová slova anglicky

copper oxide nanoparticles; immunotoxicity; immune response; lymphocytes; cytokines; inflammation; phagocytic activity and respiratory burst; antioxidant defense

Návaznosti

EF15_003/0000469, projekt VaV. EF17_043/0009632, projekt VaV. 857560, interní kód Repo. RECETOX RI, velká výzkumná infrastruktura.

Změněno: 11. 6. 2025 00:50, RNDr. Daniel Jakubík

Anotace

V originále

Copper oxide nanoparticles (CuO NPs) are increasingly used in various industry sectors. Moreover, medical application of CuO NPs as antimicrobials also contributes to human exposure. Their toxicity, including toxicity to the immune system and blood, raises concerns, while information on their immunotoxicity is still very limited. The aim of our work was to evaluate the effects of CuO NPs (number concentration 1.40x10(6) particles/cm(3), geometric mean diameter 20.4 nm) on immune/inflammatory response and antioxidant defense in mice exposed to 32.5 mu g CuO/m(3) continuously for 6 weeks. After six weeks of CuO NP inhalation, the content of copper in lungs and liver was significantly increased, while in kidneys, spleen, brain, and blood it was similar in exposed and control mice. Inhalation of CuO NPs caused a significant increase in proliferative response of T-lymphocytes after mitogenic stimulation and basal proliferative activity of splenocytes. CuO NPs significantly induced the production of IL-12p70, Th1-cytokine IFN-gamma and Th2-cytokines IL-4, IL-5. Levels of TNF-alpha and IL-6 remained unchanged. Immune assays showed significantly suppressed phagocytic activity of granulocytes and slightly decreased respiratory burst. No significant differences in phagocytosis of monocytes were recorded. The percentage of CD3(+), CD3(+)CD4(+), CD3(+)CD8(+), and CD3(-)CD19(+) cell subsets in spleen, thymus, and lymph nodes did not differ between exposed and control animals. No changes in hematological parameters were found between the CuO NP exposed and control groups. The overall antioxidant protection status of the organism was expressed by evaluation of GSH and GSSG concentrations in blood samples. The experimental group exposed to CuO NPs showed a significant decrease in GSH concentration in comparison to the control group. In summary, our results indicate that sub-chronic inhalation of CuO NPs can cause undesired modulation of the immune response. Stimulation of adaptive immunity was indicated by activation of proliferation and secretion functions of lymphocytes. CuO NPs elicited pro-activation state of Th1 and Th2 lymphocytes in exposed mice. Innate immunity was affected by impaired phagocytic activity of granulocytes. Reduced glutathione was significantly decreased in mice exposed to CuO NPs.

Přiložené soubory

https://is.muni.cz/publication/2241157/2241157.pdf

Citovat

TULINSKA, Jana, Miroslava Lehotska MIKUSOVA, Aurelia LISKOVA, Milena BUSOVA, Vlasta MASANOVA, Iveta UHNAKOVA, Eva ROLLEROVA, Radka ALACOVA, Zora KRIVOSIKOVA, Ladislava WSOLOVA, Maria DUSINSKA, Mira HORVATHOVA, Michaela SZABOVA, Norbert LUKAN, Martina STUCHLIKOVA, Daniel KUBA, Zbynek VECERA, Pavel COUFALIK, Kamil KRUMAL, Lukas ALEXA, Lucie VRLIKOVA, Marcela BUCHTOVÁ, Jana DUMKOVÁ, Pavel PILER, Vojtěch THON a Pavel MIKUSKA. Copper Oxide Nanoparticles Stimulate the Immune Response and Decrease Antioxidant Defense in Mice After Six-Week Inhalation. Frontiers in Immunology. LAUSANNE: FRONTIERS MEDIA SA, 2022, roč. 13, April 2022, s. 1-12. ISSN 1664-3224. Dostupné z: https://dx.doi.org/10.3389/fimmu.2022.874253.

@article{53253,
   author = {Tulinska, Jana and Mikusova, Miroslava Lehotska and Liskova, Aurelia and Busova, Milena and Masanova, Vlasta and Uhnakova, Iveta and Rollerova, Eva and Alacova, Radka and Krivosikova, Zora and Wsolova, Ladislava and Dusinska, Maria and Horvathova, Mira and Szabova, Michaela and Lukan, Norbert and Stuchlikova, Martina and Kuba, Daniel and Vecera, Zbynek and Coufalik, Pavel and Krumal, Kamil and Alexa, Lukas and Vrlikova, Lucie and Buchtová, Marcela and Dumková, Jana and Piler, Pavel and Thon, Vojtěch and Mikuska, Pavel},
   article_location = {LAUSANNE},
   article_number = {April 2022},
   doi = {http://dx.doi.org/10.3389/fimmu.2022.874253},
   keywords = {copper oxide nanoparticles; immunotoxicity; immune response; lymphocytes; cytokines; inflammation; phagocytic activity and respiratory burst; antioxidant defense},
   language = {eng},
   issn = {1664-3224},
   journal = {Frontiers in Immunology},
   title = {Copper Oxide Nanoparticles Stimulate the Immune Response and Decrease Antioxidant Defense in Mice After Six-Week Inhalation},
   url = {https://www.frontiersin.org/articles/10.3389/fimmu.2022.874253/full},
   volume = {13},
   year = {2022}
}

TY  - JOUR
ID  - 53253
AU  - Tulinska, Jana - Mikusova, Miroslava Lehotska - Liskova, Aurelia - Busova, Milena - Masanova, Vlasta - Uhnakova, Iveta - Rollerova, Eva - Alacova, Radka - Krivosikova, Zora - Wsolova, Ladislava - Dusinska, Maria - Horvathova, Mira - Szabova, Michaela - Lukan, Norbert - Stuchlikova, Martina - Kuba, Daniel - Vecera, Zbynek - Coufalik, Pavel - Krumal, Kamil - Alexa, Lukas - Vrlikova, Lucie - Buchtová, Marcela - Dumková, Jana - Piler, Pavel - Thon, Vojtěch - Mikuska, Pavel
PY  - 2022
TI  - Copper Oxide Nanoparticles Stimulate the Immune Response and Decrease Antioxidant Defense in Mice After Six-Week Inhalation
JF  - Frontiers in Immunology
VL  - 13
IS  - April 2022
SP  - 1-12
EP  - 1-12
PB  - FRONTIERS MEDIA SA
SN  - 1664-3224
KW  - copper oxide nanoparticles
KW  - immunotoxicity
KW  - immune response
KW  - lymphocytes
KW  - cytokines
KW  - inflammation
KW  - phagocytic activity and respiratory burst
KW  - antioxidant defense
UR  - https://www.frontiersin.org/articles/10.3389/fimmu.2022.874253/full
N2  - Copper oxide nanoparticles (CuO NPs) are increasingly used in various industry sectors. Moreover, medical application of CuO NPs as antimicrobials also contributes to human exposure. Their toxicity, including toxicity to the immune system and blood, raises concerns, while information on their immunotoxicity is still very limited. The aim of our work was to evaluate the effects of CuO NPs (number concentration 1.40x10(6) particles/cm(3), geometric mean diameter 20.4 nm) on immune/inflammatory response and antioxidant defense in mice exposed to 32.5 mu g CuO/m(3) continuously for 6 weeks. After six weeks of CuO NP inhalation, the content of copper in lungs and liver was significantly increased, while in kidneys, spleen, brain, and blood it was similar in exposed and control mice. Inhalation of CuO NPs caused a significant increase in proliferative response of T-lymphocytes after mitogenic stimulation and basal proliferative activity of splenocytes. CuO NPs significantly induced the production of IL-12p70, Th1-cytokine IFN-gamma and Th2-cytokines IL-4, IL-5. Levels of TNF-alpha and IL-6 remained unchanged. Immune assays showed significantly suppressed phagocytic activity of granulocytes and slightly decreased respiratory burst. No significant differences in phagocytosis of monocytes were recorded. The percentage of CD3(+), CD3(+)CD4(+), CD3(+)CD8(+), and CD3(-)CD19(+) cell subsets in spleen, thymus, and lymph nodes did not differ between exposed and control animals. No changes in hematological parameters were found between the CuO NP exposed and control groups. The overall antioxidant protection status of the organism was expressed by evaluation of GSH and GSSG concentrations in blood samples. The experimental group exposed to CuO NPs showed a significant decrease in GSH concentration in comparison to the control group. In summary, our results indicate that sub-chronic inhalation of CuO NPs can cause undesired modulation of the immune response. Stimulation of adaptive immunity was indicated by activation of proliferation and secretion functions of lymphocytes. CuO NPs elicited pro-activation state of Th1 and Th2 lymphocytes in exposed mice. Innate immunity was affected by impaired phagocytic activity of granulocytes. Reduced glutathione was significantly decreased in mice exposed to CuO NPs.
ER  -

TULINSKA, Jana, Miroslava Lehotska MIKUSOVA, Aurelia LISKOVA, Milena BUSOVA, Vlasta MASANOVA, Iveta UHNAKOVA, Eva ROLLEROVA, Radka ALACOVA, Zora KRIVOSIKOVA, Ladislava WSOLOVA, Maria DUSINSKA, Mira HORVATHOVA, Michaela SZABOVA, Norbert LUKAN, Martina STUCHLIKOVA, Daniel KUBA, Zbynek VECERA, Pavel COUFALIK, Kamil KRUMAL, Lukas ALEXA, Lucie VRLIKOVA, Marcela BUCHTOVÁ, Jana DUMKOVÁ, Pavel PILER, Vojtěch THON a Pavel MIKUSKA. Copper Oxide Nanoparticles Stimulate the Immune Response and Decrease Antioxidant Defense in Mice After Six-Week Inhalation. \textit{Frontiers in Immunology}. LAUSANNE: FRONTIERS MEDIA SA, 2022, roč.~13, April 2022, s.~1-12. ISSN~1664-3224. Dostupné z: https://dx.doi.org/10.3389/fimmu.2022.874253.