amp; setting A cross-sectional, mixed-design survey study, administered online and conducted with GPs from seven randomly selected Czech regions. Of the invited 777 GPs, 196 participants responded (25% response rate) and 153 completed the survey. Method Quantitative (measured using, for example, the eHealth readiness scale) and qualitative (thematic analysis) methods were used. Results Hierarchical multilinear regression (controling for age, sex, and city size) showed that ICT usage in general practice was predicted by eHealth readiness. Among GPs with their own practice, age and practice size also predicted ICT use. Analysis of barriers specific to mobile health tools identified obstacles on the side of GPs (such as low perceived usefulness), patients (such as lack of interest), and contextual barriers (such as lack of time). Conclusion In addition to system-level change, educating Czech GPs about the benefits of eHealth tools for better patient–provider interaction and quality of care is necessary to facilitate eHealth adoption and usage in the Czech Republic.