The Regulation 1143/2014 is addressing serious problems which are caused by intentional and unintentional introduction and spread of invasive alien species to the environment. While preventive restrictions imposed on individuals are aimed at intentional behavior, EU Member States are in charge with unintentional introduction and spread. It was found out that the Regulation laid down only few provisions directly applicable to individuals. The obligations related to detection, surveillance, adoption of emergency measures, eradiction and restoration of the damaged ecosystems are imposed on EU Member States. Therefore, this Regulation seems to have rather character of a directive then regulation and EU Member States have to adopt implementing legislation to ensure fulfilment of the Regulation´s requirements. The aim of this chapter was to map the current legislation to find out if it can satisfy the EU law requirements in respect to duties imposed on individuals. The conclusion can be drawn that currently the Czech legislation in force does not comply with those requirements.