J
2019
Label-free Voltammetric Detection of Products of Terminal Deoxynucleotidyl Transferase Tailing Reaction
HERMANOVA, M., P. HAVRANOVA-VIDLAKOVA, A. ONDRACKOVA, S.S. KUMAR, R. BOWATER et. al.
Základní údaje
Originální název
Label-free Voltammetric Detection of Products of Terminal Deoxynucleotidyl Transferase Tailing Reaction
Autoři
HERMANOVA, M. (203 Česká republika), P. HAVRANOVA-VIDLAKOVA (203 Česká republika), A. ONDRACKOVA (203 Česká republika), S.S. KUMAR (826 Velká Británie a Severní Irsko), R. BOWATER (826 Velká Británie a Severní Irsko) a Miroslav FOJTA (203 Česká republika, garant, domácí)
Vydání
Electroanalysis, WEINHEIM, WILEY-VCH Verlag GmbH, 2019, 1040-0397
Další údaje
Typ výsledku
Článek v odborném periodiku
Utajení
není předmětem státního či obchodního tajemství
Kód RIV
RIV/00216224:14740/19:00107289
Organizace
Středoevropský technologický institut – Masarykova univerzita – Repozitář
Klíčová slova anglicky
terminal deoxynucleotidyl transferase; oligonucleotide tailing; DNA electrochemistry; label free; nucleobase; reduction; oxidation
Návaznosti
GBP206/12/G151, projekt VaV. LQ1601, projekt VaV. 692068, interní kód Repo.
V originále
A label-free approach that takes advantage of intrinsic electrochemical activity of nucleobases has been applied to study the products of terminal deoxynucleotidyl transferase (TdT) tailing reaction. DNA homooligonucleotides A(30), C-30 and T-30 were used as primers for the tailing reaction to which a dNTP - or a mixture of dNTPs - and TdT were added to form the tails. Electrochemical detection enabled study of the tailing reaction products created by various combinations of primers and dNTPs, with pyrolytic graphite electrode (PGE) being suitable for remarkably precise analysis of the length of tailing reaction products. Furthermore, the hanging mercury drop electrode (HMDE) was able to reveal formation of various DNA structures, such as DNA hairpins and G-quadruplexes, which influence the behavior of DNA molecules at the negatively charged surface of HMDE. Thus, the described approach proves to be an excellent tool for studying the TdT tailing reactions and for exploring how various DNA structures affect both the tailing reactions and electrochemical behavior of DNA oligonucleotides at electrode surfaces.
Zobrazeno: 20. 10. 2024 02:21