Objective: The aim of the research project was to determine the influence of pain on the quality of life among patients with diseases of the musculoskeletal system. Methods: A standardized questionnaire WHOQoL-BREF by the World Health Organization was used to determine the quality of life of the patients. Furthermore, the visual analog scale (VAS) was used to determine the intensity of the pain. Altogether, there were 121 respondents to the questionnaire – the group comprised of 50 men and 71 women. The research was carried out in three locations within the Moravian-Silesian region from October 2017 to February 2018. Results: According to the results, the respondents’ quality of life showed significantly lower 65 values in the areas of physical health, experience and social relationships in comparison with the population norm. The highest average values were recorded within the area of environment (13.90 ± 3.32) in which they surpassed the standard population’s values (13,30 ± 2.08). The lowest values of the patients’ quality of life were measured for the area of physical health (11.95 ± 2.54). All respondents reported they had been in pain; the intensity of their pain varied from 2-10 and the average level of pain was 4.98 ± 1.89. It was proved that the decline of the quality of life was connected to the intensity of the pain. Conclusion: A significant deterioration in the quality of life among patients with pain and diseases of the musculoskeletal system was in the domain of physical health. On the other hand, the patients achieved better results than the healthy population in the area of environment.