This study is the first attempt to examine the validity of the Expressions of Spirituality Inventory (ESI-R) for use in the Czech context. Data were obtained via online survey from 222 emerging adults (18-25 years; 82% women), with a background in helping professions. Structural equation modelling revealed reasonably good support for structural validity of the ESI-R factor model. In addition, all of the dimension scores showed acceptable interitem consistency. However, our findings presumptively point to problems with measurement non-invariance as some items were observed to correlate with different dimensions than expected. Overall, these findings are consistent with cross-cultural research on ESI-R showing that the ESI-R has structural but not measurement invariance at the item and dimension level and provide support for further use of the ESI-R with Czech respondents.