J 2017

Directed evolution of biocircuits using conjugative plasmids and CRISPR-Cas9: design and in silico experiments

BENEŠ, David; Alfonso RODRIGUEZ-PATON and Petr SOSÍK

Basic information

Original name

Directed evolution of biocircuits using conjugative plasmids and CRISPR-Cas9: design and in silico experiments

Authors

BENEŠ, David (203 Czech Republic, guarantor, belonging to the institution); Alfonso RODRIGUEZ-PATON (724 Spain) and Petr SOSÍK (203 Czech Republic, belonging to the institution)

Edition

Natural Computing, Springer Netherlands, 2017, 1567-7818

Other information

Language

English

Type of outcome

Article in a journal

Field of Study

10201 Computer sciences, information science, bioinformatics

Country of publisher

Netherlands

Confidentiality degree

is not subject to a state or trade secret

References:

URL

RIV identification code

RIV/47813059:19240/17:A0000085

Organization

Filozoficko-přírodovědecká fakulta – Slezská univerzita v Opavě – Repository

DOI

http://dx.doi.org/10.1007/s11047-016-9595-9

UT WoS

000407824400010

EID Scopus

2-s2.0-85006414967

Keywords in English

Biocircuit; Conjugative plasmid; CRISPR-Cas9; Directed evolution; Evolutionary search; Genetic circuit; Programmed evolution

Tags

SGS132016, ÚI

Tags

International impact, Reviewed

Links

LQ1602, research and development project.
Changed: 26/3/2018 08:50, Mgr. Kamil Matula

Abstract

V originále

Recent links between computer science and synthetic biology allow for construction of many kinds of algorithmic processes within cells, obtained either by a direct engineered design or by an evolutionary search. In the latter case, horizontal gene transfer and especially transfer of plasmids by conjugation is generally respected as a crucial source of genetic diversity in bacteria. While some previous studies focused on mutations as the crucial principle to obtain diversity for engineered evolution, here we consider conjugation itself as a tool to generate diversity from a pre-determined library of biocircuits basic components. The recent development of CRISPR-Cas9 and its programmable DNA cutting ability makes it a powerful selection tool able to remove nonfunctional biocircuits from a cell population. In this paper, we describe a framework for controlled bacterial evolution of biocircuits based on conjugation and on CRISPR-Cas9, resulting in a direct biological implementation of an evolutionary algorithm. In silico experiments provide data to estimate the computational/search capability of plasmid-based engineered evolution.
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