J
2017
Directed evolution of biocircuits using conjugative plasmids and CRISPR-Cas9: design and in silico experiments
BENEŠ, David; Alfonso RODRIGUEZ-PATON and Petr SOSÍK
Basic information
Original name
Directed evolution of biocircuits using conjugative plasmids and CRISPR-Cas9: design and in silico experiments
Authors
BENEŠ, David (203 Czech Republic, guarantor, belonging to the institution); Alfonso RODRIGUEZ-PATON (724 Spain) and Petr SOSÍK (203 Czech Republic, belonging to the institution)
Edition
Natural Computing, Springer Netherlands, 2017, 1567-7818
Other information
Type of outcome
Article in a journal
Field of Study
10201 Computer sciences, information science, bioinformatics
Country of publisher
Netherlands
Confidentiality degree
is not subject to a state or trade secret
RIV identification code
RIV/47813059:19240/17:A0000085
Organization
Filozoficko-přírodovědecká fakulta – Slezská univerzita v Opavě – Repository
EID Scopus
2-s2.0-85006414967
Keywords in English
Biocircuit; Conjugative plasmid; CRISPR-Cas9; Directed evolution; Evolutionary search; Genetic circuit; Programmed evolution
Tags
International impact, Reviewed
Links
LQ1602, research and development project.
In the original language
Recent links between computer science and synthetic biology allow for construction of many kinds of algorithmic processes within cells, obtained either by a direct engineered design or by an evolutionary search. In the latter case, horizontal gene transfer and especially transfer of plasmids by conjugation is generally respected as a crucial source of genetic diversity in bacteria. While some previous studies focused on mutations as the crucial principle to obtain diversity for engineered evolution, here we consider conjugation itself as a tool to generate diversity from a pre-determined library of biocircuits basic components. The recent development of CRISPR-Cas9 and its programmable DNA cutting ability makes it a powerful selection tool able to remove nonfunctional biocircuits from a cell population. In this paper, we describe a framework for controlled bacterial evolution of biocircuits based on conjugation and on CRISPR-Cas9, resulting in a direct biological implementation of an evolutionary algorithm. In silico experiments provide data to estimate the computational/search capability of plasmid-based engineered evolution.
Displayed: 17/9/2025 09:03