Přehled o publikaci
2015
MALDI-TOF MASS SPECTROMETRY IMAGING OF METALLOTHIONEIN IN CHICKEN EMBRYO
GURÁŇ, Roman; Iva BLAZKOVA; Marketa KOMINKOVA; Ondřej ZÍTKA; René KIZEK et. al.Basic information
Original name
MALDI-TOF MASS SPECTROMETRY IMAGING OF METALLOTHIONEIN IN CHICKEN EMBRYO
Authors
GURÁŇ, Roman; Iva BLAZKOVA; Marketa KOMINKOVA; Ondřej ZÍTKA; René KIZEK and Vojtěch ADAM
Edition
Brno, MendelNet 2015 – Proceedings of International PhD Students Conference, p. 453-457, 5 pp. 2015
Publisher
Mendel University in Brno
Other information
Language
English
Type of outcome
Proceedings paper
Confidentiality degree
is not subject to a state or trade secret
References:
Organization
Repository – Repository
ISBN
978-80-7509-363-9
Keywords in English
zinc-binding proteins; MALDI-TOF MSI; chicken embryo
Changed: 2/9/2020 15:56, RNDr. Daniel Jakubík
Abstract
In the original language
In last decades the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry imaging (MALDI-TOF MSI) has become an outstanding tool for detecting spatial distribution of different biomarkers in a variety of tissue samples. It utilizes the benefits of MALDI-TOF technique, which are rapid measurements of all mass spectra in a wide mass range and detection of analytes molecular weights. Moreover, the in situ identification of targeted biomarkers can be performed too. In our study, we focused on detection of metallothionein (MT) in chicken embryo. Metallothioneins are low-molecular weight proteins connected with cancer development and protection of organism against environmental pollution. Their main functions are detoxification of heavy metals, maintaining ion homeostasis and protection against the oxidative stress. According to our knowledge, nobody has done MALDI-TOF MSI of MT so far. Therefore, we have selected MT as our studied analyte not only because of this fact but also because a part of team IGA project is aimed on MT.
