Přehled o publikaci
2025
CRISPR-BASED GENOME EDITING IN BRASSICACEAE USING TISSUE CULTURE SYSTEMS
JEDLIČKOVÁ, VeronikaBasic information
Original name
CRISPR-BASED GENOME EDITING IN BRASSICACEAE USING TISSUE CULTURE SYSTEMS
Authors
JEDLIČKOVÁ, Veronika
Edition
OP JAK TANGENC TOWARDS NEXT GENERATION CROPS: FROM DISCOVERY TO APPLICATIONS, Telč, 2025
Other information
Language
English
Type of outcome
Konferenční abstrakta
Country of publisher
Czech Republic
Confidentiality degree
is not subject to a state or trade secret
References:
Marked to be transferred to RIV
No
Organization
Středoevropský technologický institut – Repository – Repository
Keywords in English
Hairy root cultures; CRISPR; genome editing; Brassica napus; Transgene-free plants
Links
EH22_008/0004581, research and development project.
Changed: 18/3/2026 00:50, RNDr. Daniel Jakubík
Abstract
In the original language
Hairy root cultures represent a versatile system applicable to a wide range of studies, including the functional characterization of genes and their regulatory elements. In our laboratory, we have established a CRISPR-based genome editing protocol for oilseed rape (Brassica napus) using hairy roots. This transformation system offers a straightforward and rapid approach to evaluate the functionality and efficiency of diverse CRISPR editing constructs. Using an optimized regeneration protocol, we are able to produce transgene-free, genome edited plant lines. Our future objectives involve the application of CRISPR technologies for base editing, prime editing, and transcriptional regulation within the Brassicaceae family using hairy root cultures.
